Product Name |
14.3.3 beta protein |
Catalog No |
30R-1163 |
Size |
100 ug |
Concentration |
1 mg/ml |
Price |
$ 395.00
|
Synonymns |
Tyrosine 3-monocxygenase/tryptopan 5-monooxygenase activation protein, beta polypeptide protein, 14-3-3 beta protein, YWHAB protein, GW128 protein, HS1 protein, KCIP-1 protein, Tyrosine 3-monocxygenase/tryptopan 5-monooxygenase activation protein, beta polypeptide 14 3 3 protein beta/alpha protein, Brain protein 14 3 3 beta isoform protein, GW 128 protein, HS 1 protein, KCIP 1 protein, KCIP1 protein, Protein 1054 protein, Protein kinase C inhibitor protein 1 protein, Tyrosine 3 Monooxygenase/Tryptophan 5 Monooxygenase Activation Protein Beta Polypeptide. |
Description |
Purified recombinant Human 14.3.3 beta protein |
Background |
The 14-3-3 family plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, beta, gamma, epsilon,sigma, zeta, tau and eta that have been identified in mammals. The 14-3-3 beta, a subtype of the 14-3-3 proteins, was found in B Cells, brain and liver etc. This 14-3-3 beta has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Recombinant human 14-3-3 beta was expressed in E. coli and purified by using conventional chromatography techniques.
|
Species |
Human |
Residues |
1-246 amino acids: MTMDKSELVQ KAKLAEQAER YDDMAAAMKA VTEQGHELSN EERNLLSVAY KNVVGARRSS WRVISSIEQK TERNEKKQQM GKEYREKIEA ELQDICNDVL ELLDKYLIPN ATQPESKVFY LKMKGDYFRY LSEVASGDNK QTTVSNSQQA YQEAFEISKK EMQPTHPIRL GLALNFSVFY YEILNSPEKA CSLAKTAFDE AIAELDTLNE ESYK |
Molecular Weight |
28 kDa (246 aa), confirmed by MALDI-TOF. |
Protein Type |
Recombinant |
.
Grade & Purity |
> 90% by SDS - PAGE |
Form & Buffer |
Supplied as a liquid in 20mM Tris, pH 8.0, containing 1mM EDTA, and 50mM NaCl. |
Storage |
Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles. |
Applications |
SDS-PAGE |
Additional Information |
14.3.3 beta protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user. |
