Product Name |
14.3.3 eta protein (His tag) |
Catalog No |
80R-1050 |
Size |
100 ug |
Concentration |
1 mg/ml |
Price |
$ 295.00
|
Synonymns |
YWHA1 protein, YWHAH protein, Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, eta polypeptide protein, 14-3-3 eta protein, Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, eta polypeptide 14 3 3 protein eta protein, HGNC:12853 protein, Protein AS1 protein, Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein eta polypeptide protein, Tyrosine 3/tryptophan 5 monooxygenase activation protein eta polypeptide protein, YWHA 1. |
Description |
Purified recombinant Human 14.3.3 eta protein |
Background |
The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, beta, gamma, epsilon,sigma, zeta, tau and eta that have been identified in mammals. The 14-3-3eta (eta), a subtype of the 14-3-3 family of proteins, was found in B cells, brain, cerebrospinal fluid etc. 14-3-3eta interacts with and relocalizes the A20 zinc finger protein from the insoluble to the soluble fraction, suggesting a chaperone function. Recombinant human 14-3-3eta, fused to His-tag at N-terminus, was expressed in E. coli and purified by conventional chromatography techniques.
|
Species |
Human |
Residues |
1-246 amino acids: MGSSHHHHHH SSGLVPRGSH MGDREQLLQR ARLAEQAERY DDMASAMKAV TELNEPLSNE DRNLLSVAYK NVVGARRSSW RVISSIEQKT MADGNEKKLE KVKAYREKIE KELETVCNDV LSLLDKFLIK NCNDFQYESK VFYLKMKGDY YRYLAEVASG EKKNSVVEAS EAAYKEAFEI SKEQMQPTHP IRLGLALNFS VFYYEIQNAP EQACL |
Molecular Weight |
30.3 kDa (266 aa), confirmed by MALDI-TOF. |
Tag |
His tag |
Protein Type |
Recombinant |
.
Grade & Purity |
> 95% by SDS - PAGE |
Form & Buffer |
Supplied as a liquid in 20mM Tris, pH 8.0. |
Storage |
Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles. |
Applications |
SDS-PAGE |
Additional Information |
14.3.3 eta protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user. |
