Product Name |
14.3.3 sigma protein |
Catalog No |
30R-1435 |
Size |
100 ug |
Concentration |
1 mg/ml |
Price |
$ 295.00
|
Synonymns |
stratifin protein, Epithelial cell marker protein 1 protein, HME1 protein, YWHAS protein, SFN protein, 14-3-3 sigma |
Description |
Purified recombinant Human 14.3.3 sigma protein |
Background |
14-3-3 sigma, also known as Stratifin (SFN), belongs to the 14-3-3 family. The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, beta, gamma, epsilon, sigma, zeta, tau and eta that have been identified in mammals. 14-3-3 sigma was identified as an epithelial cell marker and appeared to function as a tumor suppressor whose expression can be down regulated via methylation. Loss of 14-3-3 sigma expression results in a defective G2/M phase checkpoint and appears to contribute to both epithelial and non-epithelial tumorigenesis. Recombinant human 14-3-3 sigma was expressed in E. coli and purified by using conventional chromatography techniques. |
Species |
Human |
Residues |
1-248 amino acids: MERASLIQKA KLAEQAERYE DMAAFMKGAV EKGEELSCEE RNLLSVAYKN VVGGQRAAWR VLSSIEQKSN EEGSEEKGPE VREYREKVET ELQGVCDTVL GLLDSHLIKE AGDAESRVFY LKMKGDYYRY LAEVATGDDK KRIIDSARSA YQEAMDISKK EMPPTNPIRL GLALNFSVFH YEIANSPEEA ISLAKTTFDE AMADLHTLSE DSYKD |
Molecular Weight |
27.7 kDa (248aa), confirmed by MALDI-TOF. |
Protein Type |
Recombinant |
.
Grade & Purity |
> 95% by SDS - PAGE |
Form & Buffer |
Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 50mM NaCl and 10% glycerol. |
Storage |
Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles. |
Applications |
SDS-PAGE |
Additional Information |
14.3.3 Sigma protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user. |
