Product Name |
3G11 sialoganglioside antigen antibody |
Catalog No |
10R-6528 |
Size |
50 ug |
Concentration |
500 ug/ml |
Price |
$ 125.00
|
Synonyms |
Monoclonal 3G11 sialoganglioside antigen antibody, Anti-3G11 sialoganglioside antigen antibody |
Description |
Mouse monoclonal 3G11 sialoganglioside antigen antibody |
Background |
The 3G11 monoclonal antibody reacts with the mouse 3G11antigen. The 3G11 antibody was isolated as an autoantibody against the 3G11 sialylated carbohydrate antigen, which was found to be expressed by thymoyctes and peripheral T cells, but not by B cells, myeloid cells or erythrocytes. 3G11 is expressed at higher levels on CD4+ T cells than on CD8+ T cells, however expression on both of these subsets decreases with advancing age. In thymus, 3G11 is expressed on approximately 20% of total thymocytes. 3G11 has also been used as a marker to distinguish naïve and memory T cells, as 3G11 is irreversibly down-regulated upon activation of CD4+ T cells. The 3G11 antibody is able to block binding of antibodies to CD3epsilon, suggesting close proximity of these two proteins. Recently, it has been demonstrated that loss of 3G11 expression in experimental autoimmune encephalomyelitis is associated with an anergic, regulatory T phenotype. Purified 3G11- T cells were able to suppress proliferation and cytokine production of 3G11+ T cells, and part of this effect was due to the secretion of IL-10. |
Host |
Mouse |
Isotype |
IgM kappa |
Clone |
3G11 |
Specificity |
Mouse |
Method of Purification |
3G11 sialoganglioside antigen antibody was purified by affinity chromatography. |
Form & Buffer |
Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer. |
Storage |
Store at 2-8 deg C. Do not freeze. Protect from light. |
Applications |
FC |
Usage Recommendations |
FC: = 1 ug/test |
Assay Information |
A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal /formance in the assay of interest |
