Product Name |
ARL1 protein (His tag) |
Catalog No |
80R-1422 |
Size |
100 ug |
Concentration |
0.25 mg/ml |
Price |
$ 255.00
|
Synonyms |
ARL1, ARL-1 protein, ARL-1, ARFL1 protein, ARL 1, ARL 1 protein, ADP-ribosylation factor-like protein 1 |
Description |
Purified recombinant Human ARL1 protein |
Background |
ADP-ribosylation factor-like protein 1(ARL1) belongs to the ARL (ADP-ribosylation factor-like) family of proteins, which are structurally related to ADP-ribosylation factors (ARFs). ARFs, described as activators of cholera toxin (CT) ADP-ribosyltransferase activity, regulate intracellular vesicular membrane trafficking, and stimulate a phospholipase D (PLD) isoform. Although, ARL proteins were initially thought not to activate CT or PLD, later work showed that they are weak stimulators of PLD and CT in a phospholipid dependent manner. Recombinant human ARL1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques. |
Species |
Human |
Residues |
1-181 amino acids: MGSSHHHHHH SSGLVPRGSH MGGFFSSIFS SLFGTREMRI LILGLDGAGK TTILYRLQVG EVVTTIPTIG FNVETVTYKN LKFQVWDLGG QTSIRPYWRC YYSNTDAVIY VVDSCDRDRI GISKSELVAM LEEEELRKAI LVVFANKQDM EQAMTSSEMA NSLGLPALKD RKWQIFKTSA TKGTGLDEAM EWLVETLKSR Q |
Molecular Weight |
22.5 kDa (201aa) confirmed by MALDI-TOF |
Tag |
His tag |
Protein Type |
Recombinant |
Expression System |
E.coli |
.
Grade & Purity |
> 95% by SDS - PAGE |
Form & Buffer |
Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 40% glycerol, 100mM NaCl and 2mM DTT. |
Storage |
Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles. |
Applications |
SDS-PAGE |
Additional Information |
ARL1 protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user. |
