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Cortisol in Saliva Elisa Kit  (55R-RE52061)

ELISA kit for detection of Free Cortisol in Salivain the research laboratory

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Product Name
Cortisol in Saliva Elisa Kit
Catalog No
55R-RE52061
Size
96 wells
Price
$235.00
Synonyms
Cortisol Elisa Kit, Cortisol in Saliva Elisa Kit
Description
ELISA kit for detection of Free Cortisol in Salivain the research laboratory
Background
The hormone Cortisol is vital for several functions of the human body. A strong correlation exists between stress related conditions and Cortisol levels (1–3) Cortisol is a steroid hormone made in the adrenal glands. Among its important functions in the body include roles in the regulation of blood pressure and cardiovascular function as well as regulation of the body's use of proteins, carbohydrates, and fats.
Storage
Store at 4 deg C.
Applications
ELISA
Usage Recommendations
Optimal conditions to be determined by end user
Biohazard Information
All reagents of this test kit derived from human or animal serum or plasma have been tested and confirmed negative for HIV I/II, HbsAg and HCV. However, there is no test method that can offer complete assurance therefore all reagents, should be treated as
Assay Information
The free cortisol in saliva ELISA kit is a solid phase ELISA based on the principle of competitive binding. The microtiter wells are coated with a mouse monoclonal antibody directed towards an antigenic site on the Cortisol molecule. Endogenous Cortisol of a patient sample competes with a Cortisol-horseradish peroxidase conjugate for binding to the coated antibody. After incubation the unbound conjugate is washed off. The amount of bound peroxidase conjugate is inversely proportional to the concentration of Cortisol in the sample. After addition of the substrate solution, the intensity of colour developed is reverse proportional to the concentration of Cortisol in the patient sample.