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FasL protein (30R-AF033)

Purified soluble Human FasL protein

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Product Name
FasL protein
Catalog No
30R-AF033
Size
10 ug
Price
$225.00
Synonyms
Apo I Ligand protein, CD95L protein, TNFSF6 protein, soluble Fas Ligand protein, APTL protein, sFASL protein
Description
Purified soluble Human FasL protein
Background
Fas and Fas Ligand (FasL) belong to the TNF superfamily and are type I and type II transmembrane proteins, respectively. Binding of FasL to Fas triggers apoptosis in Fas-bearing cells. The mechanism of apoptosis involves recruitment of pro-caspase 8 through an adaptor molecule called FADD followed by processing of the pro-enzyme to active forms. These active caspases then cleave various cellular substrates leading to the eventual cell death. sFasR is capable of inhibiting FasL-induced apoptosis by acting as a decoy receptor that serves as a sink for FasL. The full length Fas (receptor) is a 319 amino acid type I transmembrane protein, which contains a 157 amino acid extracellular domain, a 17 amino acid transmembrane domain, and 145 amino acid cytoplasmic domain.
Species
Human
Residues
Region of FasL protein corresponding to amino acids HHHHHHHHPS PPPEKKELRK VAHLTGKSNS RSMPLEWEDT YGIVLLSGVK YKKGGLVINE TGLYFVYSKV YFRGQSCNNL PLSHKVYMRN SKYPQDLVMM EGKMMSYCTT GQMWARSSYL GAVFNLTSAD HLYVNVSELS LVNFEESQTF FGLYKL.
Molecular Weight
17.6 kDa
Protein Type
Recombinant
Expression System
CHO cells
Grade & Purity
> 95% pure
Form & Buffer
Supplied as a lyophilized powder.
Storage
Store at -20 deg C until reconstitution. Following reconstitution product may be stored at 4 deg C in the short term. For long term storage aliquot and freeze at -20 deg C. Avoid repeated freeze/thaw cycles.
Applications
User optimized
Endotoxin Levels
Endotoxin level is less than 0.1 ng per ug (1 EU/ug).
Bioactivity
Determined by it's ability to induce cytotoxicity in Jurkat cells in the absence of any cross-linking. The ED50 for this effect is ? 10.0 ng/ml, corresponding to a specific activity of ? 1 x 10^5 units/mg.