Product Name |
AID antibody (biotin) |
Catalog No |
61R-1690 |
Size |
100 ug |
Concentration |
500 ug/ml |
Price |
$ 475.00
|
Synonyms |
Monoclonal AID antibody, Anti-AID antibody, Activation-induced cytidine deaminase antibody |
Description |
Rat monoclonal AID antibody (biotin) |
Background |
The 961 antibody reacts with mouse activation-induced cytidine deaminase, a member of the cytidine deaminase family, which was cloned from a mouse B-cell lymphoma line induced by combined stimulation with transforming growth factor-beta, interleukin-4, and CD40 ligand. The human AID gene encodes a 198-amino acid protein containing a conserved cytidine deaminase motif. The human AID protein shares 92% amino acid identity with mouse AID. RT-PCR analysis of 15 human tissues detected strong expression of AID mRNA in lymph nodes and tonsils. Class switch recombination is a region-specific DNA recombination reaction that replaces one immunoglobulin heavy-chain constant region gene with another. This enables a single variable region gene to be used in conjunction with different downstream heavy-chain constant region genes, each having a unique biologic activity. AID, a putative RNA editing enzyme, is required for this action. |
Host |
Rat |
Isotype |
IgG2a kappa |
Clone |
961 |
Specificity |
Mouse |
Form & Buffer |
Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer. |
Storage |
Store at 2-8 deg C. Do not freeze. Protect from light. |
Applications |
FC |
Usage Recommendations |
FC: = 1 ug/test |
Assay Information |
A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. |
