Product Name |
CCR7 antibody |
Catalog No |
10R-6505 |
Size |
25 ug |
Concentration |
500 ug/ml |
Price |
$ 125.00
|
Synonyms |
Monoclonal CCR7 antibody, Anti-CCR7 antibody, CCR7, C-C chemokine receptor type 7 antibody, CCR-7 antibody, CCR-7 antibody, CCR 7, MIP-3 beta Receptor antibody, CCR 7 antibody, EBI-1 antibody, CCR-7, CMKBR7 antibody |
Description |
Rat monoclonal CCR7 antibody |
Background |
The 4B12 monoclonal antibody reacts with mouse CCR7, also known as EBI-1 and CD197. For optimal visualization of CCR7 expression on different cell types it is necessary to use multi-color staining to discriminate different cell subsets. To address specificity, the staining profile of 4B12 has been compared to a polyclonal antibody generated against a CCR7 peptide. This analysis confirms that the polyclonal antibody and 4B12 stain similar populations of cells. Furthermore, 4B12 stains mouse CCR7-GFP fusion protein-transfected RBL cells. CCR7 is a chemokine receptor for the chemokines CCL19 and CCL21. In recent years, the role of chemokines in directing the migration of lymphocytes has been well-characterized. One of the most important mediators of homeostatic trafficking of naïve T cells to secondary lymphoid organs is the chemokine receptor CCR7. Binding of its ligands, CCL19 and CCL21, mediates the transendothelial migration of T cells across high endothelial venules into SLO. It has also been demonstrated that CCR7 plays a role in the localization of dendritic cells and B cells during an immune response.In addition to its significant role in the chemotaxis of lymphocytes, human CCR7 has also been recognized as a marker for a distinct subset of memory T cells, the central memory population. These cells are characterized by the expression of CCR7 and CD62L and reside within peripheral lymphoid organs. CCR7 also plays a role in thymocyte development and its deficiency leads to disturbed thymic architecture, aberrant T cell development, and limited thymocyte expansion. |
Host |
Rat |
Isotype |
IgG2a kappa |
Clone |
4B12 |
Specificity |
Mouse |
Method of Purification |
CCR7 antibody was purified by affinity chromatography. |
Form & Buffer |
Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer. |
Storage |
Store at 2-8 deg C. Do not freeze. Protect from light. |
Applications |
FC, IHC, IP, WB |
Usage Recommendations |
FC: = 0.5 ug/test |
Assay Information |
A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal /formance in the assay of interest. |
