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ETV3 antibody ( 10R-1245 )

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Product Name
ETV3 antibody
Catalog No
10R-1245
Size
50 ug
Concentration
100 ug/ml
Price
$ 190.00
Synonyms
Monoclonal ETV3 antibody, Anti-ETV3 antibody, ETV3, METS antibody, PE1 antibody, PE-1 antibody, ETV-3 antibody, bA110J1.4 antibody, ETV-3, Ets variant gene 3, ETV 3, ETV 3 antibody
Description
Mouse monoclonal ETV3 antibody
Background
Transcriptional repressor that contribute to growth arrest during terminal macrophage differentiation by repressing target genes involved in Ras-dependent proliferation. Represses MMP1 promoter activity{EC1}.
Immunogen
ETV3 antibody was raised in mouse using recombinant Ets Variant Gene 3
Host
Mouse
Isotype
IgG1
Clone
ETV3F4D10
Specificity
Human ETV3
Cross Reactivity
Human. Other species not studied
Method of Purification
ETV3 antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Form & Buffer
ETV3 antibody in PBS (3.0 mM KCl, 1.5 mM KH2 PO4 , 140 mM NaCl, 8.0 mM Na2 HPO4 (pH 7.4)) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN3 ).
Storage
Store at 2-8 deg C for up to one year. We recommend long term storage at -20 deg C. Avoid repeated freezing and thawing.
Applications
Dot Blot, ICC, WB
Usage Recommendations
WB: 0.2-2 ug/ml, ICC: 2-100 ug/ml
Biohazard Information
This antibody solution contains sodium azide (NaN ) as a preservative. There is a potential hazard that NaN reacts with copper or lead to produce an explosive compound. For safe disposal, the vial has to be washed thoroughly with water. It is strongly adv
 
Western Blot analysis of immunized recombinant protein using ETV3 antibody (10R-1245)
Immunofluorescent staining using ETV3 antibody (10R-1245)
ETV3 antibody Images:
Western Blot analysis of immunized recombinant protein using ETV3 antibody (10R-1245)
Immunofluorescent staining using ETV3 antibody (10R-1245)
Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-X100 in PBS. The cells were immunostained with anti-ETV3 antibody.