Product Name |
Caspase 9 antibody |
Catalog No |
20R-1472 |
Size |
100 ug |
Concentration |
200 ug/ml |
Price |
$ 595.00
|
Synonyms |
Polyclonal Caspase 9 antibody, Anti-Caspase 9 antibody, Caspase -9 antibody, Caspase -9, Caspase-9 antibody, Caspase 9 antibody, Cysteine-Aspartic Acid Protease 9 antibody, Caspase 9, Caspase 9 |
Description |
Rabbit polyclonal Caspase 9 antibody |
Background |
Caspases are synthesized as inactive pro enzymes that are processed to active form in cells undergoing apoptosis. Caspase 9 is an important member of the caspase family. Upon induction of apoptosis, Cytochrome C released from mitochondria associates with pro Caspase 9 (47 kDa) and Apaf 1. The complex processes pro Caspase 9 into a large subunit (37 kDa/17 kDa) and a small subunit (10 kDa). Cleaved Caspase 9 further processes other caspases including Caspase 3 and Caspase 6, to initiate a caspase cascade leading to apoptosis. The affinity purified antibody recognizing the active forms of Caspase 9 provides a new tool for identifying apoptotic cell populations in both tissue sections and cultured cells. |
Immunogen |
Caspase 9 antibody was raised in Rabbit using synthetic peptide mapping to the N-terminus adjacent to Asp330 of human caspase-9 as the immunogen. |
Host |
Rabbit |
Specificity |
The anti-active Caspase-9 antibody recognizes only the cleaved Caspase-9 (37 kDa). It does not recognize full-length Caspase-9 or any other caspases. |
Cross Reactivity |
Human |
Method of Purification |
Caspase 9 antibody was purified by affinity purification. |
Form & Buffer |
Supplied in PBS buffer containing 50% glycerol, 0.5% BSA, and 0.02% thimerosal. |
Storage |
Store at -20 deg C. For long-term storage, aliquot and freeze at -70 deg C. Avoid repeated freeze/thaw cycles. |
Applications |
ICC, IP, WB |
Usage Recommendations |
IP: 10-20 ug/ml, ICC: 10-20 ug/ml, WB: 0.5-4 ug/ml |
