Product Name |
13(s)-HODE ELISA kit |
Catalog No |
55R-IB09603 |
Size |
96 tests |
Price |
$ 595.00
|
Synonymns |
13(s)-HODE ELISA kit, 13-hydroxyoctadecadienoic acid ELISA kit |
Description |
ELISA kit for the detection of HODE in the research laboratory |
Background |
The major human dietary polyunsaturated fatty acid is linoleic acid, an essential fatty acid that can be metabolized to either 13-hydroxyoctadecadienoic acid via the lipoxygenase [13(S)HODE] or P450 [13(R)HODE] pathways or to 15-hydroxyeicosatetraenoic acid [15(S)HETE] via the cyclooxygenase pathway. Changes in the intracellular ratio of 13(S)HODE to 15(S)HETE have been linked to a number of physiological conditions dominated by cancer and cardiovascular diseases. 13(S)HODE helps to mediate cellular adhesion properties and excessive proliferation that is critical in the formation of tumors, metastasis and artherogenic plaques. Continuously synthesized in nonstimulated vessel epithelia, 13(S)HODE remains associated with the vitronectin receptor, an ubiquitous integrin adhesion molecule. Cell surfaces become adhesive when HODE concentrations decline, allowing the vitronectin receptor to relocate. |
Storage |
Store at 2-8 deg C |
Applications |
ELISA |
Usage Recommendations |
Optimal conditions to be determined by end user |
Assay Information |
This 13hydroxyoctadecadienoic acid [13(S)HODE] Enzyme Immunoassay (EIA) kit is a competitive immunoassay for the quantitative determination of free 13(S)HODE in biological fluids. Please read the complete kit insert before performing this assay. The kit uses a polyclonal antibody to 13(S)HODE to bind, in a competitive manner, the 13(S)HODE in the sample or an alkaline phosphatasemoleculewhich has 13(S)HODEcovalentlyattached to it. After a simultaneous incubation at room temperature the excess reagents are washed away and substrate is added. After another incubation, the enzyme reaction is stopped and the yellowcolor generated read on amicroplate reader at 405 nm. The intensity of the bound yellowcolor is inversely proportional to the concentration of 13(S)HODE in either standards or samples. The measured optical density is used to calculate the concentrationof13(S)HODE. |
