Product Name |
Adrenaline/Epinephrine ELISA kit |
Catalog No |
55R-IB89171 |
Size |
96 tests |
Price |
$ 535.00
|
Synonyms |
Adrenaline ELISA kit, Epinephrine ELISA kit |
Description |
ELISA kit for the detection of Adrenalin/Epinephrine in the research laboratory |
Background |
Epinephrine (also known as adrenaline) is a hormone and a neurotransmitter. It increases heart rate, constricts blood vessels, dilates air passages and participates in the fight-or-flight response of the sympathetic nervous system. Chemically, epinephrine is a catecholamine, a monoamine produced only by the adrenal glands from the amino acids phenylalanine and tyrosine. |
Storage |
Store at 2-8 deg C. |
Applications |
ELISA |
Usage Recommendations |
Optimal conditions to be determined by end-user |
Assay Information |
Adrenaline (epinephrine) is extracted by using a cisdiolspecific affinity gel, acylated and then derivatized enzymatically. The competitive ELISA kit uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The derivatized standards, controls and samples and the solid phase bound analytes compete for a fixed number of antiserum binding sites. After the system is in equilibrium, free antigen and free antigenantiserum complexes are removed by washing. The antibody bound to the solid phase is detected by an antirabbit IgGperoxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm. Determination of unknown samples is achieved by comparing their absorbance with a reference curve prepared with known standard concentrations. |
