Product Name |
beta 2 Microglobulin ELISA kit |
Catalog No |
55R-ORG5BM |
Size |
96 tests |
Price |
$ 215.00
|
Synonyms |
beta-2-Microglobulin ELISA kit, B2M ELISA kit, |
Description |
ELISA kit for the detection of beta 2 Microglobulin in the research laboratory |
Background |
Proteins passing the glomerular basal membrane of the kidney undergo differentiated filtering. The permeability is inversly proportional to the molecular weight (Albumin about 0.6%, Myoglobulin about 75%). Nevertheless, only minimal quantities of protein are detectable in urine, because large quantities of protein are reabsorbed by the tubuli. Elevated glomerular protein permeability and high tubular plasma protein elimination can be differentiated by measuring the molecular weight distribution of the eliminated proteins. Albumin has a relative molecular mass of 66,000 Da. It is contained in urine at very low concentrations. In case of a very active glomerular filtering process the albumin secretion can arise without an underlying kidney disease. This situation is called "Microalbuminuria". The detection of these small secretion quantities (30 to 150 ug/min or ml) requires very sensitive test systems, i.e. immunological techniques. Physical stress can induce elevated albumin secretion too, without the occurence of a kidney disease. In Diabetes, Albumin secretion is a very important parameter for the evaluation of the kidney function. Urine values higher than 25 ug/ml indicate a detrimental kidney function of insulin-dependent (type I) and noninsulin-dependent (type II) diabetic patients. The determination of Albumin is therefore an important diagnostic tool in diabetic nephropathies. |
Storage |
Store at 2-8 deg C |
Applications |
ELISA |
Usage Recommendations |
Optimal conditions to be determined by end-user |
Assay Information |
beta2Microglobulin is an indirect solid phase enzyme immunometric assay (ELISA). It is designed for the quantitative measurement of beta2Microglobulin in human urine, serum or plasma samples. The microplate is coated with highly purified antihBeta2Microglobulin antibodies. The plate can be divided into 12 modules of 8 wells each or can be used completely for 96 determinations. Each well can be separated from the module ("breakaway"). The binding of the analyte, the formation of the sandwich complexes and the enzymatic color reaction take place during three different reaction phases. The amount of colour is directly proportional to the concentration of beta2Microglobulin present in the original sample. To read the optical density a microplate reader with a 450 nm filter is required. Bichromatic measurement with a 600 690 nm reference is recommended. |
