Product Name |
Chicken anti Mouse IgG (H + L) (Alk Phos) |
Catalog No |
43R-1299 |
Size |
500 ug |
Concentration |
1.50 mg/ml |
Price |
$ 105.00
|
Synonyms |
Chicken anti Mouse Immunoglobulin G (H + L) secondary antibody (Alk Phos) |
Description |
Chicken anti Mouse IgG (H + L) secondary antibody (Alk Phos) |
Background |
Immunoglobulin G (IgG) are antibody molecules. Each IgG is composed of four peptide chains - two heavy chains and two light chains. Each IgG has two antigen binding sites. Other Immunoglobulins may be described in terms of polymers with the IgG structure considered the monomer. IgG constitutes 75% of serum immunoglobulins in humans. IgG molecules are synthesized and secreted by plasma B cells. |
Immunogen |
Purified Mouse IgG (H&L) |
Host |
Chicken |
Specificity |
This antibody reacts with heavy (gamma) chains on mouse IgG and light chains on all mouse immunoglobulins. |
Cross Reactivity |
No reactivity is observed to non-immunoglobulin mouse serum proteins or light chains on all human immunoglobulins or IgG from human or rabbit or serum proteins from human or rabbit. |
Grade & Purity |
> 95% based on SDS-PAGE |
Method of Purification |
Affinity purified using solid phase Mouse IgG (H&L) |
Source |
Ig fraction was prepared using eggs obtained from healthy hens of US origin. |
Form & Buffer |
Clear, colorless liquid, containing 30 mM Triethanolamine, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1% (w/v) BSA and 0.05% (w/v) Sodium Azide. Protease/IgG free, pH 7.2. |
Storage |
Store undiluted liquid at 2-8 deg C. For storage at -20 deg C, aliquot and dilute with an equal volume of glycerol to prevent loss of enzymatic activity. |
Applications |
User optimized |
Usage Recommendations |
1:5000-1:50,000 ELISA/WB, 1:500-1:2000 IHC |