Product Name |
Chicken anti Mouse IgG (H + L) (FITC) |
Catalog No |
43R-1295 |
Size |
1 mg |
Concentration |
1.0 mg/ml |
Price |
$ 125.00
|
Synonyms |
Chicken anti Mouse Immunoglobulin G (H + L) secondary antibody (FITC) |
Description |
Chicken anti Mouse IgG (H + L) secondary antibody (FITC) |
Background |
Immunoglobulin G (IgG) are antibody molecules. Each IgG is composed of four peptide chains - two heavy chains and two light chains. Each IgG has two antigen binding sites. Other Immunoglobulins may be described in terms of polymers with the IgG structure considered the monomer. IgG constitutes 75% of serum immunoglobulins in humans. IgG molecules are synthesized and secreted by plasma B cells. |
Immunogen |
Purified Mouse IgG (H&L) |
Host |
Chicken |
Specificity |
This antibody reacts with heavy (gamma) chains on mouse IgG and light chains on all mouse immunoglobulins. |
Cross Reactivity |
No reactivity is observed to non-immunoglobulin mouse serum proteins. |
Grade & Purity |
> 95% based on SDS-PAGE |
Method of Purification |
Affinity purified using solid phase Mouse IgG (H&L) |
Source |
Ig fraction was prepared using eggs obtained from healthy hens of US origin. |
Form & Buffer |
Clear, fluorescent yellow liquid, containing 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, 1% (w/v) BSA and 0.05% (w/v) Sodium Azide. Protease/IgG free, pH 7.2. |
Storage |
Store at 2-8 deg C. |
Applications |
FC, IF, IHC |
Usage Recommendations |
The optimal working dilution should be determined by the end user. Suggested starting dilution(s): 1:20 – 1:2000 for most applications |
