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Tuberculosis IgM ELISA kit (55R-IB79285)

ELISA kit for the detection of Tuberculosis IgM in the research laboratory

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Product Name
Tuberculosis IgM ELISA kit
Catalog No
96 wells
Tuberculosis IgM ELISA kit
ELISA kit for the detection of Tuberculosis IgM in the research laboratory
Mycobacterioses (tuberculosis, leprosy, atypical mycobacterioses, paratuberculosis, and perhaps Crohn’s Disease) are the infectious diseases of men and animals with the largest diffusion on earth. The infectious agents of tuberculosis are acid-resistant rod-like formed bacteria of the family Mycobacteriaceae, genus Mycobacterium. The germ was detected by Robert Koch in 1882. Owing to the very high infectious power of pathogenic mycobacteria, early diagnosis is essential to prevent spreading of the disease. Convergence of various approaches are necessary to control the mycobacterioses, immune reactions and bacterial shedding being variable during the diseases. However, usual diagnostic procedures were up to now unsatisfactory and did not allow us to distinguish among different mycobacterial species. The illness is normally transferred by droplets of saliva from infected persons. The target of the infection are mostly the lungs, but also other organs like the brain, intestinal tract, bones, lymph nodes and kidneys can be afflicted. Tuberculosis is not only found in developing countries with 8 million new infections yearly, but also in industrialized civilizations, as an actual disease with some thousands of cases yearly. Without treatment, the disease leads in 50% of the cases to death within less than two years. Clinical symptoms are fatigue, loss of weight, lack of appetite, light fever, nocturnal sweat and pain in the chest. Especially patients with HIV are threatened by tuberculosis due to their impaired immune system. A vaccination with living attenuated bacteria is possible (BCG = Bacille Calmette Guérin). This is mostly done with newborn or young children. With older patients, before the vaccination the tuberculin test is usually performed (Pirquet or Mantoux), where a small amount of tuberculin is injected under the skin. In a positive case, there exist antibodies against Mycobacteria, and a vaccination is not necessary. Up to recently, there have not existed any serological methods to detect tuberculosis antibodies in serum. The only available procedure was besides the skin tuberculin test the direct microscopical identification of the dyed bacteria in sputum. Meanwhile specific antigens have been prepared either by purification of natural material or by recombinant methods. This ELISA test kit for the determination of IgG antibodies uses a cocktail of highly pure proteins in order to determine an immune response against the bacteria in human serum. A fresh or chronically active infection can be diagnosed by IgA and IgM tests, which are also available.
Store at 2-8 deg C
Usage Recommendations
Optimal conditions to be determined by end user
Assay Information
This Mycobacterium tuberculosis IgM antibody test kit is based on the principle of the enzyme immunoassay (EIA). Mycobacterium tuberculosis antigen is bound on the surface of the microtiter strips. Diluted patient serum or readytouse standards are pipetted into the wells of the microtiter plate. A binding between the IgM antibodies of the serum and the immobilized Mycobacterium tuberculosis antigen takes place. After a one hour incubation at room temperature, the plate is rinsed with diluted wash solution, in order to remove unbound material. Then readytouse antihumanIgM peroxidase conjugate is added and incubated for 30 minutes. After a further washing step, the substrate (TMB) solution is pipetted and incubated for 20 minutes, inducing the development of a blue dye in the wells. The color development is terminated by the addition of a stop solution, which changes the color from blue to yellow. The resulting dye is measured spectrophotometrically at the wavelength of 450 nm. The concentration of IgM antibodies is directly proportional to the intensity of the color. NOTE that samples containing hyperimmune levels of antihuman IgG antibodies may cause false positive results in this assay. Any patient samples testing positive should be prediluted with IgG adsorbent and retested to confirm elevated levels of IgM.