14.3.3 sigma protein (30R-1435)
Purified recombinant Human 14.3.3 sigma protein
|Synonyms||HME1 protein, SFN protein, 14.3.3 sigma, Epithelial cell marker protein 1 protein, stratifin protein, 14-3-3 sigma protein, 14/3/3 sigma protein, YWHAS protein, 14-3-3 sigma, 14/3/3 sigma, 14-3-3 sigma|
Coomassie Blue stained SDS-PAGE of 14.3.3 sigma protein (30R-1435)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-248 amino acids: MERASLIQKA KLAEQAERYE DMAAFMKGAV EKGEELSCEE RNLLSVAYKN VVGGQRAAWR VLSSIEQKSN EEGSEEKGPE VREYREKVET ELQGVCDTVL GLLDSHLIKE AGDAESRVFY LKMKGDYYRY LAEVATGDDK KRIIDSARSA YQEAMDISKK EMPPTNPIRL GLALNFSVFH YEIANSPEEA ISLAKTTFDE AMADLHTLSE DSYKDSTLIM QLLRDNLTLW TADNAGEEGG EAPQEPQS|
|Purity||> 95% pure|
|Molecular Weight||27.7 kDa (248aa), confirmed by MALDI-TOF.|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 50mM NaCl and 10% glycerol.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||14-3-3 sigma, also known as Stratifin (SFN), belongs to the 14-3-3 family. The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, beta, gamma, epsilon, sigma, zeta, tau and eta that have been identified in mammals. 14-3-3 sigma was identified as an epithelial cell marker and appeared to function as a tumor suppressor whose expression can be down regulated via methylation. Loss of 14-3-3 sigma expression results in a defective G2/M phase checkpoint and appears to contribute to both epithelial and non-epithelial tumorigenesis. Recombinant human 14-3-3 sigma was expressed in E. coli and purified by using conventional chromatography techniques.|
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