14.3.3 zeta protein (His tag) (80R-1064)

Purified recombinant Human 14.3.3 zeta protein

Synonyms Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein protein, Tyr-3/Trp-5 monooxygenase Activation protein protein, Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein protein, zeta polypeptide protein, Phospholipase A2 protein, Zeta, KCIP-1 protein, 14/3/3 zeta protein, 14-3-3 zeta, Protein kinase C inhibitor protein 1 protein, MGC111427 protein, 14-3-3 zeta protein, 14/3/3 zeta, 14.3.3 zeta, Tyr3/Trp5 monooxygenase Activation protein protein, 14-3-3 zeta protein, zeta polypeptide 14 3 3 protein zeta/delta protein, KCIP1 protein, YWHAZ protein, Zeta protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

Coomassie Blue stained SDS-PAGE of 14.3.3 zeta protein (His tag) (80R-1064)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 1-245 amino acids: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMDK NELVQKAKLA EQAERYDDMA ACMKSVTEQG AELSNEERNL LSVAYKNVVG ARRSSWRVVS SIEQKTEGAE KKQQMAREYR EKIETELRDI CNDVLSLLEK FLIPNASQAE SKVFYLKMKG DYYRYLAEVA AGDDKKGIVD QSQQAYQEAF EISKKEMQPT HPIRLGLALN FSVFYYEILN SPEKACSLAK TAFDEAIAEL DTLSEESYKD STLIMQLLRD NLTLWTSDTQ GDEAEAGEGG EN
Expression System E.coli
Purity > 95% pure
Molecular Weight 32 kDa (245 aa), confirmed by MALDI-TOF.
Tag/Conjugate His tag
Form & Buffer Supplied as a liquid in PBS, pH 7.4.
Concentration 1 mg/ml

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, beta, gamma, epsilon,sigma, zeta, tau and eta that have been identified in mammals. 14-3-3 zeta interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Recombinant human YWHAZ, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

References
Add a Paper

Chimen M, McGettrick HM, Apta B, Kuravi SJ, Yates CM, Kennedy A, Odedra A, Alassiri M, Harrison M, Martin A, Barone F, Nayar S, Hitchcock JR, Cunningham AF, Raza K, Filer A, Copland DA, Dick AD, Robinson J, Kalia N, Walker LS, Buckley CD, Nash GB, Narendran P, Rainger GE

Homeostatic regulation of T cell trafficking by a B cell–derived peptide is impaired in autoimmune and chronic inflammatory disease

Nature Medicine

Volume: 21 Issue: 5 Page: 467–475 PMID: 25894827 DOI: 10.1038/nm.3842

Reviews

You need to be logged in to write a review. Please login here

Sorry there are currently no reviews for this product

Images

  • Coomassie Blue stained SDS-PAGE of 14.3.3 zeta protein (His tag) (80R-1064) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: $255.00
Size: 100 ug
OR
Shipping
View Our Distributors