4EBP1 antibody (10R-1950)
Mouse monoclonal 4EBP1 antibody
|Synonyms||Monoclonal 4EBP1 antibody, Anti-4EBP1 antibody, 4EBP 1 antibody, 4EBP-1, Eukaryotic translation initiation factor 4E-binding protein 1 antibody, 4EBP 1, 4EBP1, BP-1 antibody, 4E BP1 antibody, MGC4316 antibody, 4E-BP1 antibody, 4EBP-1 antibody|
|Immunogen||4EBP1 antibody was raised in Mouse using a purified recombinant fragment of 4EBP1 expressed in E. coli as the immunogen.|
Western Blot analysis using 4EBP1 antibody (10R-1950)
Western Blot showing 4E-BP1 antibody used against truncated 4E-BP1 recombinant protein (1) and A431 cell lysate (2).
|Form & Buffer||Supplied as a liquid in ascitic fluid containing 0.03% sodium azide.|
Usage & Assay Information
|Usage Recommendations||ELISA: 1:10,000, WB: 1:500-1:2000|
Storage & Safety
|Storage||Store at 4 deg C for short term storage. Store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||4EBP1 which is located on chromosome 8p12, with 118-amino acid protein (about 13kDa). Binding of eIF4EBP1 to eIF4E is reversible and is dependent on the phosphorylation status of eIF4EBP1. Non phosphorylated eIF4EBP1 will bind strongly to eIF4E while the phosphorylated form will not. Akt, TOR, MAP kinase, S6 kinase, and Cdc2 are known kinases capable of inactivating eIF4EBP1 binding to eIF4E by phosphorylating either threonines 35, 45, 69 or serine 64. 4EBP1 functions as a translation repressor.|
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