5-Lipoxygenase protein (30R-AL007)

Purified recombinant Human 5-Lipoxygenase protein

Synonyms 5-Lipoxygenase Antigen, 5 Lipoxygenase Recombinant Protein
Species Human
Protein Type Recombinant
Applications User optimized

Specifications

Expression System Baculovirus
Molecular Weight 78 kDa
Form & Buffer 50mM Tris containing 5mM EDTA, pH 8.0.
Concentration Batch dependent - please inquire should you have specific requirements

Usage & Assay Information

Bioactivity 20 units/mg protein - One unit is defined as the amount of enzyme that consumes 1.0 nmol of oxygen per min at 25 DEG C in 50 mM Tris-HCl, 100 uM arachidonate, 2 mM CaCl2, 1 mM ATP, 250 uM oxygen. Oxygen consumption can be measured using a oxygraph equippe

Storage & Safety

Storage Aliquot and store at -70 deg C or lower. Avoid repeated freeze/thaw cycles

General Information

Biological Significance 5-lipoxygenase (5-LO) catalyzes the formation of 5(S)-HpETE from arachidonic acid and its subsequent conversion to leukotriene A4. It is localized in the cytosol of resting human and rat peripheral blood neutrophils, whereas it is found in the nucleus in rat basophilic leukemia cells and human alveolar macrophages. Upon stimulation of the cell, 5-LO translocates to the nuclear membrane where it is found in association with 5-LO activating protein (FLAP) and with the 85 kDa cPLA2.

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Availability: In stock

Price: $445.00
Size: 500 units
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