ACAA1 protein (His tag) (80R-1436)
Purified recombinant Human ACAA1 protein
|Synonyms||ACAA-1 protein, peroxisomal, ACAA-1, ACAA 1, ACAA1, 3-ketoacyl-CoA thiolase protein, PTHIO protein, ACAA 1 protein, ACAA protein, THIO protein|
Coomassie Blue stained SDS-PAGE of ACAA1 protein (His tag) (80R-1436)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||27-424 amino acids: MGSSHHHHHH SSGLVPRGSH MLSGAPQASA ADVVVVHGRR TAICRAGRGG FKDTTPDELL SAVMTAVLKD VNLRPEQLGD ICVGNVLQPG AGAIMARIAQ FLSDIPETVP LSTVNRQCSS GLQAVASIAG GIRNGSYDIG MACGVESMSL ADRGNPGNIT SRLMEKEKAR DCLIPMGITS ENVAERFGIS REKQDTFALA SQQKAARAQS KGCFQAEIVP VTTTVHDDKG TKRSITVTQD EGIRPSTTME GLAKLKPAFK KDGSTTAGNS SQVSDGAAAI LLARRSKAEE LGLPILGVLR SYAVVGVPPD IMGIGPAYAI PVALQKAGLT VSDVDIFEIN EAFASQAAYC VEKLRLPPEK VNPLGGAVAL GHPLGCTGAR QVITLLNELK RRGKRAYGVV SMCIGTGMGA AAVFEYPGN|
|Purity||> 95% pure|
|Molecular Weight||43.8 kDa (419aa) confirmed by MALDI-TOF|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 10% glycerol, 1mM DTT, and 0.1M NaCl.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||ACAA1 is a member of the thiolase family of enzymes and is involved in lipid metabolism. This protein is localized to the peroxisome and catalyzes the conversion of acyl-CoA and acetyl-CoA to 3-oxoacyl-CoA in the fatty acid oxidation pathway. ACAA1 shows high enzymatic activity in liver, kidney, intestine and white adipose tissue in rats. Deficiency of this enzyme leads to pseudo-Zellweger syndrome. Recombinant human ACAA1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.|
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