ACADS protein (His tag) (80R-1230)
Purified recombinant Human ACADS protein
|Synonyms||SCAD protein, Acyl-Coenzyme A dehydrogenase protein, Bcd-1 protein, short chain protein, mitochondrial., C2 to C3 short chain protein, Acyl-Coenzyme A dehydrogenase Acyl Coenzyme A dehydrogenase protein, Unsaturated acyl CoA reductase protein, Short-chain specific acyl-CoA dehydrogenase protein, Bcd1 protein, ACAD3 protein, short chain protein, C2 to C3 short chain protein, Short chain acyl CoA dehydrogenase protein, Butyryl CoA dehydrogenase protein, Acyl-CoA dehydrogenase protein, AI196007 protein, Acyl-CoA dehydrogenase protein, EC 22.214.171.124 protein|
Coomassie Blue stained SDS-PAGE of ACADS protein (His tag) (80R-1230)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||25-412 amino acids: MGSSHHHHHH SSGLVPRGSH MLHTIYQSVE LPETHQMLLQ TCRDFAEKEL FPIAAQVDKE HLFPAAQVKK MGGLGLLAMD VPEELGGAGL DYLAYAIAME EISRGCASTG VIMSVNNSLY LGPILKFGSK EQKQAWVTPF TSGDKIGCFA LSEPGNGSDA GAASTTARAE GDSWVLNGTK AWITNAWEAS AAVVFASTDR ALQNKSISAF LVPMPTPGLT LGKKEDKLGI RGSSTANLIF EDCRIPKDSI LGEPGMGFKI AMQTLDMGRI GIASQALGIA QTALDCAVNY AENRMAFGAP LTKLQVIQFK LADMALALES ARLLTWRAAM LKDNKKPFIK EAAMAKLAAS EAATAISHQA IQILGGMGYV TEMPAERHYR DARITEIYEG TSEIQRLVIA GHLLRSYRS|
|Purity||> 95% pure|
|Molecular Weight||44.0 kDa (409aa), confirmed by MALDI-TOF.|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 1mM DTT, 0.1 M NaCl and 20% glycerol.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||ACADS (Acyl-Coenzyme A dehydrogenase), also known as SCAD or ACAD3, is a tetrameric mitochondrial flavoprotein, which is a member of the acyl-CoA dehydrogenase family. This enzyme catalyzes the initial step of the mitochondrial fatty acid beta-oxidation pathway. Mutations of ACADS have been associated with fatty acid oxidation defects and metabolic diseases such as short-chain acyl-CoA dehydrogenase deficiency (SCAD deficiency). Recombinant human ACADS protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.|
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