ACAT2 antibody (10R-3143)

Mouse monoclonal ACAT2 antibody

Synonyms Monoclonal ACAT2 antibody, Anti-ACAT2 antibody, Acetyl-Coenzyme A Acetyltransferase 2 antibody, ACAT2, ACAT 2, ACAT-2, ACAT 2 antibody, ACAT-2 antibody
Cross Reactivity Human, Dog, Rat, Monkey
Applications FC, IF, IHC
Immunogen ACAT2 antibody was raised in mouse using a full length recombinant protein of human ACAT2 (NP_005882) produced in HEK293T cells, as the immunogen.

Images

Flow Cytometric analysis using ACAT2 antibody (10R-3143)

Flow Cytometric analysis of HEK293T cells transfected with either recombinant ACAT2 protein (red) or empty vector (blue) stained using ACAT2 antibody

Specifications

Host Mouse
Clone 3 E2
Isotype IgG2b
Method of Purification ACAT2 antibody was purified by affinity chromatography.
Form & Buffer Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Concentration 500 ug-1 mg/ml

Usage & Assay Information

Usage Recommendations FC: 1:100, IF: 1:100, IHC: 1:50, WB: 1:1000-2000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Acetyl-Coenzyme A acetyltransferase 2 is an enzyme involved in lipid metabolism. Reported patients with ACAT2 deficiency have shown severe mental retardation and hypotonus. The ACAT2 gene shows complementary overlapping with the 3-prime region of the TCP1 gene in both mouse and human. These genes are encoded on opposite strands of DNA, as well as in opposite transcriptional orientation.

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Images

  • Flow Cytometric analysis using ACAT2 antibody (10R-3143) | Flow Cytometric analysis of HEK293T cells transfected with either recombinant ACAT2 protein (red) or empty vector (blue) stained using ACAT2 antibody
  • Western Blot analysis using ACAT2 antibody (10R-3143) | Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant ACAT2 protein (Right) or empty vector (Left) detected with ACAT2 antibody
  • Immunohistochemical staining using ACAT2 antibody (10R-3143) | Immunohistochemical analysis of ACAT2 protein in paraffin embedded Human prostate tissue using ACAT2 antibody
  • Immunofluorescent staining using ACAT2 antibody (10R-3143) | Immunofluorescent staining of COS7 cells transiently transfected with recombinant ACAT2 protein using ACAT2 antibody

Availability: In stock

Price: $395.00
Size: 100 ul
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