ACAT2 antibody (10R-3146)

Mouse monoclonal ACAT2 antibody

Synonyms Monoclonal ACAT2 antibody, Anti-ACAT2 antibody, Acetyl-Coenzyme A Acetyltransferase 2 antibody, ACAT2, ACAT 2, ACAT-2, ACAT 2 antibody, ACAT-2 antibody
Cross Reactivity Human, Dog, Rat
Applications FC, IF, IHC
Immunogen ACAT2 antibody was raised in mouse using a full length recombinant protein of human ACAT2 (NP_005882) produced in HEK293T cells, as the immunogen.

Images

Flow Cytometric analysis using ACAT2 antibody (10R-3146)

Flow Cytometric analysis of HEK293T cells transfected with either recombinant ACAT2 protein (red) or empty vector (blue) stained using ACAT2 antibody

Specifications

Host Mouse
Clone 5A2
Isotype IgG1
Method of Purification ACAT2 antibody was purified by affinity chromatography.
Form & Buffer Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Concentration 500 ug-1 mg/ml

Usage & Assay Information

Usage Recommendations FC: 1:100, IF: 1:100, IHC: 1:50, WB: 1:1000-2000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Acetyl-Coenzyme A acetyltransferase 2 is an enzyme involved in lipid metabolism. Reported patients with ACAT2 deficiency have shown severe mental retardation and hypotonus. The ACAT2 gene shows complementary overlapping with the 3-prime region of the TCP1 gene in both mouse and human. These genes are encoded on opposite strands of DNA, as well as in opposite transcriptional orientation.

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Images

  • Flow Cytometric analysis using ACAT2 antibody (10R-3146) | Flow Cytometric analysis of HEK293T cells transfected with either recombinant ACAT2 protein (red) or empty vector (blue) stained using ACAT2 antibody
  • Western Blot analysis using ACAT2 antibody (10R-3146) | Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant ACAT2 protein (Right) or empty vector (Left) detected with ACAT2 antibody
  • Immunohistochemical staining using ACAT2 antibody (10R-3146) | Immunohistochemical analysis of ACAT2 protein in paraffin embedded Human Kidney tissue using ACAT2 antibody
  • Immunofluorescent staining using ACAT2 antibody (10R-3146) | Immunofluorescent staining of COS7 cells transiently transfected with recombinant ACAT2 protein using ACAT2 antibody

Availability: In stock

Price: $395.00
Size: 100 ul
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