Acid phosphatase 1 protein (His tag) (80R-1124)

Purified recombinant Human Acid phosphatase 1 protein

Synonyms LMW-PTP protein, PTPase protein, soluble isoform b Acid phosphatase 1 soluble protein, Acid phosphatase 1 protein, Adipocyte acid phosphatase Cytoplasmic phosphotyrosyl protein phosphatase protein, Red cell acid phosphatase 1 protein, Red cell acid phosphatase 1., ACP1 protein, Protein tyrosine phosphatase protein, Low molecular weight phosphotyrosine protein phosphatase protein, Purple acid phosphatase protein, PAP2 protein, Acid phosphatase of erythrocyte protein, Acid phosphatase 1 protein, Adipocyte acid phosphatase protein, HAAP protein, soluble isoform b protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

Coomassie Blue stained SDS-PAGE of Acid phosphatase 1 protein (His tag) (80R-1124)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 1-158 amino acids: MGSSHHHHHH SSGLVPRGSH MAEQATKSVL FVCLGNICRS PIAEAVFRKL VTDQNISENW VIDSGAVSDW NVGRSPDPRA VSCLRNHGIH TAHKARQITK EDFATFDYIL CMDESNLRDL NRKSNQVKTC KAKIELLGSY DPQKQLIIED PYYGNDSDFE TVYQQCVRCC RAFLEKAH
Expression System E.coli
Purity > 95% pure
Molecular Weight 20.1 kDa (178 aa), confirmed by MALDI-TOF.
Tag/Conjugate His tag
Form & Buffer Supplied as a liquid in 20mM MES, pH 6.0, containing 0.1mM PMSF, 2mM EDTA, and 10% glycerol.
Concentration 1 mg/ml

Usage & Assay Information

Bioactivity Activity tested: > 60,000 unit/mg of protein.

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.
Endotoxin Levels < 1.0 EU per ug of protein (determined by LAL method)

General Information

Biological Significance Acid phosphatase 1, soluble, also known as ACP1, belongs to the phosphotyrosine protein. It functions as an acid phosphatase and a protein tyrosine phosphatase (PTPase) present in all human tissues, including adipocytes. This enzyme hydrolyzes protein tyrosine phosphate to protein tyrosine and orthophosphate, and also orthophosphoric monoesters to alcohol and orthophosphate. Recombinant ACP1, fused to His-tag at N-terminus, was expressed in E. coli and purified by conventional chromatography techniques.

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Images

  • Coomassie Blue stained SDS-PAGE of Acid phosphatase 1 protein (His tag) (80R-1124) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: $395.00
Size: 100 ug
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