ACIN1 antibody (10R-1308)

Mouse monoclonal ACIN1 antibody

Synonyms Monoclonal ACIN1 antibody, Anti-ACIN1 antibody, ACIN1, ACIN-1, DKFZp667N107 antibody, ACIN 1, ACIN-1 antibody, ACINUS antibody, ACIN 1 antibody, KIAA0670 antibody, ACN antibody, Apoptotic chromatin condensation inducer 1 antibody
Specificity Human ACIN1
Cross Reactivity Human. Other species not studied
Applications Dot Blot, ICC, WB
Immunogen ACIN1 antibody was raised in mouse using recombinant Human Apoptotic Chromatin Condensation Inducer 1 (Acin1)

Images

Immunofluorescent staining using ACIN1 antibody (10R-1308)

Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-X100 in PBS. The cells were immunostained with anti-ACIN1 antibody.

Specifications

Host Mouse
Clone 2005C3a
Isotype IgG1
Method of Purification ACIN1 antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Form & Buffer ACIN1 antibody in PBS (3.0 mM KCl, 1.5 mM KH2 PO4 , 140 mM NaCl, 8.0 mM Na2 HPO4 (pH 7.4)) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN3 ).
Concentration 100 ug/ml

Usage & Assay Information

Usage Recommendations WB: 0.2-2 ug/ml, ICC: 2-100 ug/ml

Storage & Safety

Storage Store at 2-8 deg C for up to one year. We recommend long term storage at -20 deg C. Avoid repeated freezing and thawing.
Biohazard Information This antibody solution contains sodium azide (NaN ) as a preservative. There is a potential hazard that NaN reacts with copper or lead to produce an explosive compound. For safe disposal, the vial has to be washed thoroughly with water. It is strongly adv

General Information

Biological Significance ACIN1 is a component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Induces apoptotic chromatin condensation after activation by CASP3.

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Images

  • Immunofluorescent staining using ACIN1 antibody (10R-1308) | Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-X100 in PBS. The cells were immunostained with anti-ACIN1 antibody.
  • Western Blot analysis of immunized recombinant protein using ACIN1 antibody (10R-1308) | Left: ACIN1 staining. Right: Coomassie Blue staining of immunized recombinant protein.

Availability: In stock

Price: $190.00
Size: 50 ug
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