ACLY antibody (10R-3149)

Mouse monoclonal ACLY antibody

Synonyms Monoclonal ACLY antibody, Anti-ACLY antibody, Atp Citrate Lyase antibody, ACL antibody, ATPCL antibody, CLATP antibody
Cross Reactivity Human
Applications IF, IHC, WB
Immunogen ACLY antibody was raised in mouse using a full length recombinant protein of human ACLY (NP_001087) produced in HEK293T cells, as the immunogen.

Images

Immunofluorescent staining using ACLY antibody (10R-3149)

Immunofluorescent staining of COS7 cells transiently transfected with recombinant ACLY protein using ACLY antibody

Specifications

Host Mouse
Clone 3G8
Isotype IgG1
Method of Purification ACLY antibody was purified by affinity chromatography.
Form & Buffer Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Concentration 500 ug-1 mg/ml

Usage & Assay Information

Usage Recommendations IF: 1:100, IHC: 1:50, WB: 1:2000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance ACLY is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440, 000) of apparently identical subunits.

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Images

  • Immunofluorescent staining using ACLY antibody (10R-3149) | Immunofluorescent staining of COS7 cells transiently transfected with recombinant ACLY protein using ACLY antibody
  • Western Blot analysis using ACLY antibody (10R-3149) | Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant ACLY protein (Right) or empty vector (Left) detected with ACLY antibody
  • Immunohistochemical staining using ACLY antibody (10R-3149) | Immunohistochemical analysis of ACLY protein in paraffin embedded Human colon tissue using ACLY antibody

Availability: In stock

Price: $455.00
Size: 100 ul
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