ACO2 antibody (10R-3150)

Mouse monoclonal ACO2 antibody

Synonyms Monoclonal ACO2 antibody, Anti-ACO2 antibody, Aconitase 2 Mitochondrial antibody, ACONM antibody, MGC20605 antibody, MGC33908 antibody, ACO2, ACO 2, ACO-2, ACO 2 antibody, ACO-2 antibody
Cross Reactivity Human
Applications IF, IHC, WB
Immunogen ACO2 antibody was raised in mouse using a full length recombinant protein of human ACO2 (NP_001089) produced in HEK293T cells, as the immunogen.

Images

Immunofluorescent staining using ACO2 antibody (10R-3150)

Immunofluorescent staining of COS7 cells transiently transfected with recombinant ACO2 protein using ACO2 antibody

Specifications

Host Mouse
Clone 3G8
Isotype IgG1
Method of Purification ACO2 antibody was purified by affinity chromatography.
Form & Buffer Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Concentration 500 ug-1 mg/ml

Usage & Assay Information

Usage Recommendations IF: 1:100, IHC: 1:50, WB: 1:2000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance ACO2 belongs to the aconitase/IPM isomerase family. It is an enzyme that catalyzes the interconversion of citrate to isocitrate via cis-aconitate in the second step of the TCA cycle. This protein is encoded in the nucleus and functions in the mitochondrion. It was found to be one of the mitochondrial matrix proteins that are preferentially degraded by the serine protease 15(PRSS15), also known as Lon protease, after oxidative modification.

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Images

  • Immunofluorescent staining using ACO2 antibody (10R-3150) | Immunofluorescent staining of COS7 cells transiently transfected with recombinant ACO2 protein using ACO2 antibody
  • Western Blot analysis using ACO2 antibody (10R-3150) | Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant ACO2 protein (Right) or empty vector (Left) detected with ACO2 antibody
  • Immunohistochemical staining using ACO2 antibody (10R-3150) | Immunohistochemical analysis of ACO2 protein in paraffin embedded Human kidney tissue using ACO2 antibody

Availability: In stock

Price: $395.00
Size: 100 ul
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