ACTB antibody (10R-1242)

Mouse monoclonal ACTB antibody

Synonyms Monoclonal ACTB antibody, Anti-ACTB antibody, Actin beta antibody, PS1TP5BP1 antibody
Specificity Human ACTB
Cross Reactivity Human, Mouse, Rat. Other species not studied
Applications Dot Blot, FC, ICC, WB
Immunogen ACTB antibody was raised in mouse using recombinant Human Beta Actin

Images

Flow Cytometric assay using ACTB antibody (10R-1242)

HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-ACTB antibody (shaded) or isotype control (unshaded) followed by Alexa Fluor 488 conjugated goat anti-mouse IgG.

Specifications

Host Mouse
Clone ACTBD11B7
Isotype IgG1
Method of Purification ACTB antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Form & Buffer ACTB antibody in PBS (3.0 mM KCl, 1.5 mM KH2 PO4 , 140 mM NaCl, 8.0 mM Na2 HPO4 (pH 7.4)) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN3 ).
Concentration 100 ug/ml

Usage & Assay Information

Usage Recommendations WB: 0.2-2 ug/ml, FC: 0.5-2 ug/sample, ICC: 2-100 ug/ml

Storage & Safety

Storage Store at 2-8 deg C for up to one year. We recommend long term storage at -20 deg C. Avoid repeated freezing and thawing.
Biohazard Information This antibody solution contains sodium azide (NaN ) as a preservative. There is a potential hazard that NaN reacts with copper or lead to produce an explosive compound. For safe disposal, the vial has to be washed thoroughly with water. It is strongly adv

General Information

Biological Significance Beta actin is one of six different actin isoforms which have been identified. ACTB is one of the two nonmuscle cytoskeletal actins. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Alpha actins are a major constituent of the contractile apparatus.

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Images

  • Flow Cytometric assay using ACTB antibody (10R-1242) | HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-ACTB antibody (shaded) or isotype control (unshaded) followed by Alexa Fluor 488 conjugated goat anti-mouse IgG.
  • Western Blot analysis of immunized recombinant protein using ACTB antibody (10R-1242)
  • Immunofluorescent staining using ACTB antibody (10R-1242) | Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-X100 in PBS. The cells were immunostained with anti-ACTB antibody.

Availability: In stock

Price: $190.00
Size: 50 ug
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