ACTN1 antibody (10R-3172)

Mouse monoclonal ACTN1 antibody

Synonyms Monoclonal ACTN1 antibody, Anti-ACTN1 antibody, Actinin alpha 1 antibody, Alpha-actinin cytoskeletal isoform antibody, Non-muscle alpha actinin 1 antibody, F-actin cross linking protein antibody, Actinin 1 smooth muscle antibody, FLJ40884 antibody, ACTN1, ACTN 1, ACTN-1, ACTN 1 antibody, ACTN-1 antibody
Cross Reactivity Human
Applications IF, IHC, WB
Immunogen ACTN1 antibody was raised in mouse using a human recombinant protein fragment corresponding to amino acids 650-893 of human alpha-actinin (NP_001093) produced in E. coli as the immunogen.

Images

Immunofluorescent staining using ACTN1 antibody (10R-3172)

Immunofluorescent staining of Hela cells transiently transfected with recombinant ACTN1 protein using ACTN1 antibody

Specifications

Host Mouse
Clone 7A4
Isotype IgG2a
Method of Purification ACTN1 antibody was purified by affinity chromatography.
Form & Buffer Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Concentration 500 ug-1 mg/ml

Usage & Assay Information

Usage Recommendations IF: 1:100, IHC: 1:50, WB: 1:2000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments.

References
Add a Paper

Sorry, but there are no references currently for this product.

Reviews

You need to be logged in to write a review. Please login here

Sorry there are currently no reviews for this product

Images

  • Immunofluorescent staining using ACTN1 antibody (10R-3172) | Immunofluorescent staining of Hela cells transiently transfected with recombinant ACTN1 protein using ACTN1 antibody
  • Western Blot analysis using ACTN1 antibody (10R-3172) | Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant ACTN1 protein (Right) or empty vector (Left) detected with ACTN1 antibody
  • Immunohistochemical staining using ACTN1 antibody (10R-3172) | Immunohistochemical analysis of ACTN1 protein in paraffin embedded Human Kidney tissue using ACTN1 antibody

Availability: In stock

Price: $455.00
Size: 100 ul
OR
Shipping
View Our Distributors