ACYP1 protein (His tag) (80R-1686)
Purified recombinant Human ACYP1 protein
|Synonyms||ACYP 1 protein, ACYPE protein, ACYP 1, ACYP-1 protein, ACYP-1, erythrocyte acylphosphatase, Acylphosphatase-1 protein, ACYP1|
Coomassie Blue stained SDS-PAGE of ACYP1 protein (His tag) (80R-1686)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-99 amino acids: MGSSHHHHHH SSGLVPRGSH MGSMAEGNTL ISVDYEIFGK VQGVFFRKHT QAEGKKLGLV GWVQNTDRGT VQGQLQGPIS KVRHMQEWLE TRGSPKSHID KANFNNEKVI LKLDYSDFQI VK|
|Purity||> 90% pure|
|Molecular Weight||13.6 kDa (122aa), confirmed by MALDI-TOF|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 20% glycerol, 0.1M NaCl and 1mM DTT.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||ACYP1, also known as erythrocyte acylphosphatase, is a cytosolic enzyme that catalyzes the hydrolysis of the carboxyl-phosphate bond of acylphosphates. Two acylphophatase isoenzymes exist: ACYP1 and ACYP2. The two isoenzymes share 60% homology and have the same substrate specificity, although ACYP1 has higher catalytic activity than ACYP2. Recombinant human ACYP1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.|
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