ADAT2 protein (His tag) (80R-1471)
Purified recombinant Human ADAT2 protein
|Synonyms||ADAT-2, ADAT 2, tRNA-specific adenosine deaminase 2 protein, ADAT-2 protein, DEADC1 protein, ADAT 2 protein, TAD2, ADAT2|
Coomassie Blue stained SDS-PAGE of ADAT2 protein (His tag) (80R-1471)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-191 amino acids: MGSSHHHHHH SSGLVPRGSH MEAKAAPKPA ASGACSVSAE ETEKWMEEAM HMAKEALENT EVPVGCLMVY NNEVVGKGRN EVNQTKNATR HAEMVAIDQV LDWCRQSGKS PSEVFEHTVL YVTVEPCIMC AAALRLMKIP LVVYGCQNER FGGCGSVLNI ASADLPNTGR PFQCIPGYRA EEAVEMLKTF YKQENPNAPK SKVRKKECQK S|
|Purity||> 90% pure|
|Molecular Weight||23.2 kDa (211aa), confirmed by MALDI-TOF|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 10% glycerol, 2mM DTT and 50mM NaCl.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||Adenosine deaminase (ADA), also known as ADAT2 is an enzyme involved in purine metabolism. It is needed for the breakdown of adenosine from food and for the turnover of nucleic acids in tissues. Also, ADAT2 is thought to participate in the deamination of adenosine-34 to inosine in many tRNAs. Belonging to the cytidine and deoxycytidylate deaminase protein family, ADAT2 employs zinc as a cofactor. ADAT2 is a 191 amino acid protein that exists as two isoforms produced by alternative splicing events. Recombinant human ADAT2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.|
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