ADSL protein (His tag) (80R-2028)

Recombinant human ADSL protein (His tag)

Synonyms ASL protein, Adenylosuccinate lyase protein, AMPS protein, ASASE protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

Coomassie Blue stained SDS-PAGE of ADSL protein (His tag) (80R-2028)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 1-484 amino acids: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMAAG GDHGSPDSYR SPLASRYASP EMCFVFSDRY KFRTWRQLWL WLAEAEQTLG LPITDEQIQE MKSNLENIDF KMAAEEEKRL RHDVMAHVHT FGHCCPKAAG IIHLGATSCY VGDNTDLIIL RNALDLLLPK LARVISRLAD FAKERASLPT LGFTHFQPAQ LTTVGKRCCL WIQDLCMDLQ NLKRVRDDLR FRGVKGTTGT QASFLQLFEG DDHKVEQLDK MVTEKAGFKR AFIITGQTYT RKVDIEVLSV LASLGASVHK ICTDIRLLAN LKEMEEPFEK QQIGSSAMPY KRNPMRSERC CSLARHLMTL VMDPLQTASV QWFERTLDDS ANRRICLAEA FLTADTILNT LQNISEGLVV YPKVIERRIR QELPFMATEN IIMAMVKAGG SRQDCHEKIR VLSQQAASVV KQEGGDNDLI ERIQVDAYFS PIHSQLDHLL DPSSFTGRAS QQVQRFLEEE VYPLLKPYES VMKVKAELCL
Expression System E.coli
Purity > 95% pure
Molecular Weight 59 kDa (520aa)
Tag/Conjugate His tag
Form & Buffer Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 40% glycerol, 0.1M NaCl
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations ADSL protein (His tag) has been used in SDS-PAGE, and may be suitable for use in other applications, to be determined by the end user.

Storage & Safety

Storage Can be stored at 4 deg C short term (1-2 weeks). For long term storage, aliquot and store at -20 deg C or -70 deg C. Avoid repeated freezing and thawing cycles.

General Information

Biological Significance Adenylosuccinate lyase, also known as ADSL, is an enzyme that converts adenylosuccinate to AMP and fumarate as part of the purine nucleotide cycle. Defects in ADSL are the cause of adenylosuccinase deficiency (ADSL deficiency). ADSL deficiency is an autosomal recessive disorder characterized by the accumulation in the body fluids of succinylaminoimidazole-carboxamide riboside (SAICA-riboside) and succinyladenosine (S-Ado). Recombinant human ADSL protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

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Images

  • Coomassie Blue stained SDS-PAGE of ADSL protein (His tag) (80R-2028) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: $295.00
Size: 100 ug
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