AES protein (His tag) (80R-1712)

Purified recombinant Human AES protein

Synonyms TLE5 protein, Amino-terminal enhancer of split protein, AES-2 protein, ESP1 protein, AES-1 protein, GRG; GRG5 protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

Coomassie Blue stained SDS-PAGE of AES protein (His tag) (80R-1712)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 1-197 amino acids: MGSSHHHHHH SSGLVPRGSH MMFPQSRHSG SSHLPQQLKF TTSDSCDRIK DEFQLLQAQY HSLKLECDKL ASEKSEMQRH YVMYYEMSYG LNIEMHKQAE IVKRLNGICA QVLPYLSQEH QQQVLGAIER AKQVTAPELN SIIRQQLQAH QLSQLQALAL PLTPLPVGLQ PPSLPAVSAG TGLLSLSALG SQAHLSKEDK NGHDGDTHQE DDGEKSD
Expression System E.coli
Purity > 95% pure
Molecular Weight 24.1 kDa (217aa) confirmed by MALDI-TOF
Tag/Conjugate His tag
Form & Buffer Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 1mM DTT and 20% glycerol.
Concentration 1 mg/ml

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Amino-terminal enhancer of split, also known AES, belongs to the groucho/TLE family of proteins, can function as a homooligomer or as a heteroologimer with other family members to dominantly repress the expression of other family member genes. This protein is expressed predominately in fetal brain, liver, lung, heart and kidney and in adult muscle. In addition, AES can repress NFkB-regulated gene expression and is thought to play an important role in initiating and maintaining cell differentiation events. Recombinant human AES protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

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Images

  • Coomassie Blue stained SDS-PAGE of AES protein (His tag) (80R-1712) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: $255.00
Size: 50 ug
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