aFGF protein (30R-1075)
Purified recombinant Human aFGF protein
|Synonyms||HBGF-1 protein, Heparin binding growth factor 1 precursor., GLIO703 protein, HBGF 1 protein, HBGF1 protein, FGF alpha protein, ECGFA protein, ECGF-beta protein, AFGF protein, Endothelial Cell Growth Factor alpha protein, ECGFB protein, FGF 1 protein, Acidic Fibroblast Growth Factor (aFGF) protein, FGFA protein, Heparin binding growth factor 1 protein, Heparin-binding growth factor 1 protein, Fibroblast growth factor 1 protein, FGF1 protein, Acidic fibroblast growth factor protein, Acidic Fibroblast Growth Factor Acidic fibroblast growth factor protein, Beta endothelial cell growth factor protein, ECGF beta protein, Beta-endothelial cell growth factor protein, ECGF protein, Fibroblast Growth Factor 1 Acidic protein, aFGF protein, Endothelial Cell Growth Factor beta protein|
Coomassie Blue stained SDS-PAGE of aFGF protein (30R-1075)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||MFNLPPGNYK KPKLLYCSNG GHFLRILPDG TVDGTRDRSD QHIQLQLSAE SVGEVYIKST ETGQYLAMDT DGLLYGSQTP NEECLFLERL EENHYNTYIS KKHAEKNWFV GLKKNGSCKR GPRTHYGQKA ILFLPLPVSS D|
|Purity||> 95% pure|
|Molecular Weight||15.9 kDa (141aa), confirmed by MALDI-TOF.|
|Form & Buffer||Supplied as a liquid in 20mM Tris, pH 7.0, containing 100mM NaCl.|
Usage & Assay Information
|Bioactivity||ED50 for this effect is < 2.9 ng/ml, measured in a cell proliferation assay using NIH-3T3 cell (which corresponds to > 340,000 units/ml).|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||Acidic fibroblast growth factor (aFGF) belongs to the fibroblast growth factor family. It is a potent mitogenic agent. aFGF has been detected in large amounts in the brain. Other cells known to express FGF acidic include hepatocytes, vascular smooth muscle cells, CNS neurons, skeletal muscle cells, fibroblasts, keratinocytes, endothelial cells, intestinal columnar epithelium cells and pituitary basophils and acidophils. The growth factor acts through activation of specific cell-surface receptors leading to intracellular tyrosine phosphorylation cascade. Recombinant human aFGF was overexpressed in E. coli and purified by conventional chromatography.|
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