AFP antibody (10R-3208)

Mouse monoclonal AFP antibody

Synonyms Monoclonal AFP antibody, Anti-AFP antibody, alpha Fetoprotein antibody, alpha-Fetoprotein antibody
Cross Reactivity Human
Applications FC, IF, WB
Immunogen AFP antibody was raised in mouse using a full length recombinant protein of human AFP (NP_001125) produced in HEK293T cells, as the immunogen.

Images

Flow Cytometric analysis using AFP antibody (10R-3208)

Flow Cytometric analysis of HEK293T cells transfected with either recombinant AFP protein (red) or empty vector (blue) stained using AFP antibody

Specifications

Host Mouse
Clone 2 E2
Isotype IgG2a
Method of Purification AFP antibody was purified by affinity chromatography.
Form & Buffer Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Concentration 500 ug-1 mg/ml

Usage & Assay Information

Usage Recommendations IF: 1:100, FC: 1:100, WB: 1:500

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Alpha-fetoprotein (AFP) is a protein that in humans is encoded by the AFP gene. Alpha-fetoprotein is a major plasma protein produced by the yolk sac and the liver during fetal life. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem on chromosome 4.

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Images

  • Flow Cytometric analysis using AFP antibody (10R-3208) | Flow Cytometric analysis of HEK293T cells transfected with either recombinant AFP protein (red) or empty vector (blue) stained using AFP antibody
  • Western Blot analysis using AFP antibody (10R-3208) | Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant AFP protein (Right) or empty vector (Left) detected with AFP antibody
  • Immunofluorescent staining using AFP antibody (10R-3208) | Immunofluorescent staining of COS7 cells transiently transfected with recombinant AFP protein using AFP antibody

Availability: In stock

Price: $395.00
Size: 100 ul
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