AFP antibody (10R-3215)

Mouse monoclonal AFP antibody

Synonyms Monoclonal AFP antibody, Anti-AFP antibody, alpha Fetoprotein antibody, alpha-Fetoprotein antibody
Cross Reactivity Human, Dog
Applications IF, WB
Immunogen AFP antibody was raised in mouse using a human recombinant protein fragment corresponding to amino acids 19-397 of human AFP (NP_001125) produced in E. coli as the immunogen.

Images

Immunofluorescent staining using AFP antibody (10R-3215)

Immunofluorescent staining of Hela cells transiently transfected with recombinant AFP protein using AFP antibody

Specifications

Host Mouse
Clone 5H2
Isotype IgG1
Method of Purification AFP antibody was purified by affinity chromatography.
Form & Buffer Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Concentration 500 ug-1 mg/ml

Usage & Assay Information

Usage Recommendations IF: 1:100, WB: 1:500-1000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Alpha-fetoprotein (AFP) is a protein that in humans is encoded by the AFP gene. Alpha-fetoprotein is a major plasma protein produced by the yolk sac and the liver during fetal life. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem on chromosome 4.

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Images

  • Immunofluorescent staining using AFP antibody (10R-3215) | Immunofluorescent staining of Hela cells transiently transfected with recombinant AFP protein using AFP antibody
  • Western Blot analysis using AFP antibody (10R-3215) | Western blot analysis of HepG2 cell lysate using AFP antibody at 1:1000

Availability: In stock

Price: $395.00
Size: 100 ul
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