AGR 2 protein (His tag) (80R-1025)
Purified recombinant Human AGR 2 protein
|Synonyms||HAG-2 protein, AGR-2 protein, Anterior gradient 2 homolog protein, AG2 protein, Anterior Gradient Protein 2 Homolog, AGR 2 protein, XAG-2 protein, AGR 2, anterior gradient homolog 2 (Xenopus laevis) protein, GOB-4 protein, protein disulfide isomerase family A member 17 protein, PDIA17 protein, AGR2 protein, AGR-2, AGR 2 protein, AGR2, Anterior gradient 2 homolog protein|
Coomassie Blue stained SDS-PAGE of AGR 2 protein (His tag) (80R-1025)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||21-175 amino acids: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMRDT TVKPGAKKDT KDSRPKLPQT LSRGWGDQLI WTQTYEEALY KSKTSNKPLM IIHHLDECPH SQALKKVFAE NKEIQKLAEQ FVLLNLVYET TDKHLSPDGQ YVPRIMFVDP SLTVRADITG RYSNRLYAYE PADTALLLDN MKKALKLLKT EL|
|Purity||> 95% pure|
|Molecular Weight||22 kDa (192 aa), confirmed by MALDI-TOF.|
|Form & Buffer||Supplied as a liquid in 20mM Tris, pH 8.0, containing 1mM DTT, 10% glycerol, and 1mM EDTA.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Endotoxin Levels||< 1.0 EU per ug of protein (determined by LAL method)|
|Biological Significance||AGR2 (Anterior gradient 2 homolog) is the human orthologue of the secreted Xenopus laevis Anterior Gradient protein (XAG-2). This is a small, possibly secreted molecule of yet weakly defined functions that is widely expressed in human tissues. Expression of AGR2 shows a positive correlation with expression of estrogen receptor in breast carcinoma and a negative correlation with expression of EGF receptor. Recombinant human AGR2, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.|
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