AIDA protein (His tag) (80R-2681)

Purified recombinant AIDA protein (His tag)

Synonyms Axin interactor protein, dorsalization-associated protein, C1orf80 protein, RP11-378J18.7 protein, AIDA
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

SDS-PAGE analysis of AIDA protein (His tag) (80R-2681)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 1-306 aa: MGSSHHHHHH SSGLVPRGSH MGSMSEVTRS LLQRWGASFR RGADFDSWGQ LVEAIDEYQI LARHLQKEAQ AQHNNSEFTE EQKKTIGKIA TCLELRSAAL QSTQSQEEFK LEDLKKLEPI LKNILTYNKE FPFDVQPVPL RRILAPGEEE NLEFEEDEEE GGAGAGSPDS FPARVPGTLL PRLPSEPGMT LLTIRIEKIG LKDAGQCIDP YITVSVKDLN GIDLTPVQDT PVASRKEDTY VHFNVDIELQ KHVEKLTKGA AIFFEFKHYK PKKRFTSTKC FAFMEMDEIK PGPIVIELYK KPTDFKRKKL QLLTKKPLYL HLHQTLHKE
Expression System E.coli
Purity > 90% pure
Molecular Weight 37.4kDa
Tag/Conjugate His tag
Form & Buffer Supplied in liquid form in 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol 0.2M NaCl, 1mM DTT
Concentration 250 ug/ml

Storage & Safety

Storage Can be stored at 4 deg C for short terms (1-2 weeks). For long term storage, aliquot and store at -20 deg C or -70 deg C. Avoid repeated freezing and thawing cycles

General Information

Biological Significance AIDA acts as a ventralizing factor during embryogenesis. This protein inhibits axin-mediated JNK activation by binding axin and disrupting axin homodimerization. This in turn antagonizes a Wnt/beta-catenin-independent dorsalization pathway activated by AXIN/JNK-signaling. Recombinant human AIDA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

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Images

  • <div><font face=Calibri size=2>SDS-PAGE analysis of AIDA protein (His tag) (80R-2681)</font></div>     | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: $270.00
Size: 20 ug
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