AK1 antibody (10R-3228)

Mouse monoclonal AK1 antibody

Synonyms Monoclonal AK1 antibody, Anti-AK1 antibody, Adenylate Kinase 1 antibody, AK1, AK 1, AK-1, AK 1 antibody, AK-1 antibody
Cross Reactivity Human
Applications FC, IF, WB
Immunogen AK1 antibody was raised in mouse using a full length recombinant protein of human AK1 (NP_000467) produced in HEK293T cells, as the immunogen.

Images

Flow Cytometric analysis using AK1 antibody (10R-3228)

Flow Cytometric analysis of HEK293T cells transfected with either recombinant AK1 protein (red) or empty vector (blue) stained using AK1 antibody

Specifications

Host Mouse
Clone 18C7
Isotype IgG1
Method of Purification AK1 antibody was purified by affinity chromatography.
Form & Buffer Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Concentration 500 ug-1 mg/ml

Usage & Assay Information

Usage Recommendations FC: 1:100, IF: 1:100, WB: 1:1000-2000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Adenylate kinase is an enzyme involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate group among adinine nucleotides. Three isozymes of adenylate kinase have been identified in vertebrates, adenylate isozyme 1 (AK1), 2 (AK2) and 3 (AK3). AK1 is found in the cytosol of skeletal muscle, brain and erythrocytes, whereas AK2 and AK3 are found in the mitochondria of other tissues including liver and heart. AK1 was identified because of its association with a rare genetic disorder causing nonspherocytic hemolytic anemia where a mutation in the AK1 gene was found to reduce the catalytic activity of the enzyme.

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Images

  • Flow Cytometric analysis using AK1 antibody (10R-3228) | Flow Cytometric analysis of HEK293T cells transfected with either recombinant AK1 protein (red) or empty vector (blue) stained using AK1 antibody
  • Western Blot analysis using AK1 antibody (10R-3228) | Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant AK1 protein (Right) or empty vector (Left) detected with AK1 antibody
  • Immunofluorescent staining using AK1 antibody (10R-3228) | Immunofluorescent staining of Hela cells transiently transfected with recombinant AK1 protein using AK1 antibody

Availability: In stock

Price: $395.00
Size: 100 ul
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