AK3 antibody (10R-3232)

Mouse monoclonal AK3 antibody

Synonyms Monoclonal AK3 antibody, Anti-AK3 antibody, adenylate kinase antibody, Ak6 antibody, FIX antibody, Ak3L1 antibody, AkL3L antibody, AkL3L1 antibody, adenylate kinase 3 antibody, adenylate kinase 6 antibody, adenylate kinase 3 like 1 antibody, AK3, AK 3, AK-3, AK 3 antibody, AK-3 antibody
Cross Reactivity Human
Applications FC, WB
Immunogen AK3 antibody was raised in mouse using a full length recombinant protein of human AK3 (NP_057336) produced in HEK293T cells, as the immunogen.

Images

Flow Cytometric analysis using AK3 antibody (10R-3232)

Flow Cytometric analysis of HEK293T cells transfected with either recombinant AK3 protein (red) or empty vector (blue) stained using AK3 antibody

Specifications

Host Mouse
Clone 5A1
Isotype IgG1
Method of Purification AK3 antibody was purified by affinity chromatography.
Form & Buffer Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Concentration 500 ug-1 mg/ml

Usage & Assay Information

Usage Recommendations FC: 1:100, WB: 1:500-2000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Adenylate kinase is a ubiquitous monomeric enzyme involved energy metabolism of prokaryotic and eukaryotic cells. Three isozymes (Ak1, Ak2 and Ak3) are characterized in vertebrates. Ak1 is present in the cytosol of skeletal muscle, brain, and erythrocyte, while Ak2 is localized in the intermembrane space of mitochondria of liver, kidney, spleen and heart. Ak3, called GTP:AMP phosphotransferase, exists in the mitochondrial matrix of liver and heart. These isozymes contribute to homeostasis of the adenine nucleotide composition in the cell.

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Images

  • Flow Cytometric analysis using AK3 antibody (10R-3232) | Flow Cytometric analysis of HEK293T cells transfected with either recombinant AK3 protein (red) or empty vector (blue) stained using AK3 antibody
  • Western Blot analysis using AK3 antibody (10R-3232) | Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant AK3 protein (Right) or empty vector (Left) detected with AK3 antibody

Availability: In stock

Price: $395.00
Size: 100 ul
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