AK4 protein (His tag) (80R-1639)
Purified recombinant Human AK4 protein
|Synonyms||AK3L1 protein, AK 4, AK3L2 protein, AK3 protein, AK4, AK-4, MGC166959, Adenylate kinase 4 protein, AK-4 protein, AK 4 protein|
Coomassie Blue stained SDS-PAGE of AK4 protein (His tag) (80R-1639)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-223 amino acids: MGSSHHHHHH SSGLVPRGSH MASKLLRAVI LGPPGSGKGT VCQRIAQNFG LQHLSSGHFL RENIKASTEV GEMAKQYIEK SLLVPDHVIT RLMMSELENR RGQHWLLDGF PRTLGQAEAL DKICEVDLVI SLNIPFETLK DRLSRRWIHP PSGRVYNLDF NPPHVHGIDD VTGEPLVQQE DDKPEAVAAR LRQYKDVAKP VIELYKSRGV LHQFSGTETN KIWPYVYTLF SNKITPIQSK EAY|
|Purity||> 90% pure|
|Molecular Weight||27.4 kDa (243aa), confirmed by MALDI-TOF (Molecular size on SDS-PAGE will appear higher)|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 10% glycerol, 2mM DTT and 0.1M NaCl.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||AK4(adenylate kinase 4) is a member of the adenylate kinase family of enzymes. This protein is localized to the mitochondrial matrix. Adenylate kinases regulate the adenine and guanine nucleotide compositions within a cell by catalyzing the reversible transfer of phosphate group among these nucleotides. Inherited mutations leading to AK4 deficiencies in erythrocytes have been implicated in hemolytic anemia. Recombinant human AK4 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.|
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