AKR1C1 protein (His tag) (80R-1267)

Purified recombinant Human AKR1C1 protein

Synonyms AKRC 1, Aldo keto reductase family 1 member C1 protein, AKRC-1, DDH protein, Chlordecone reductase homolog protein, Dihydrodiol dehydrogenase 1 protein, Chlordecone reductase homolog HAKRC protein, , DD1/DD2 protein, AK1C1 protein, C9 protein, 2 diol dehydrogenase protein, AKRC 1 protein, MBAB protein, Trans-1 protein, HAKRC protein, DDH1 protein, 20 alpha hydroxysteroid dehydrogenase protein, HBAB protein, member C1 20 alpha (3 alpha) hydroxysteroid dehydrogenase protein, 20-alpha-HSD protein, AKRC-1 protein, 2 dihydrobenzene 1 protein, DD1 protein, 2ALPHAHSD protein, Aldo-keto reductase family 1 protein, H37 protein, High affinity hepatic bile acid-binding protein protein, Hepatic dihydrodiol dehydrogenase protein, AKR1C1, Dihydrodiol dehydrogenase 1/2 protein, Type II 3 alpha hydroxysteroid dehydrogenase protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE
Assay Information 1. Prepare a 1ml reaction mix into a suitable container: The final concentrations are 0.1M sodium phosphate (pH 7.0), 10mM DL-glyceraldehyde, 0.3mM NADPH. 2. Add 50ul of recombinant AKR1C1 solution with various concentrations (2.5ug, 5ug, 10ug) in 750ul reaction buffer. 3. Mix by inversion and Incubate at 25 DEG C for 2.5 minutes. 4. Add 200ul of 50 mM DL-glyceraldehyde as a substrate and immediately mix by inversion. 5. Record the increase at A340nm for 3 minutes.

Images

Coomassie Blue stained SDS-PAGE of AKR1C1 protein (His tag) (80R-1267)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 1-323 amino acids: MGSSHHHHHH SSGLVPRGSH MDSKYQCVKL NDGHFMPVLG FGTYAPAEVP KSKALEATKL AIEAGFRHID SAHLYNNEEQ VGLAIRSKIA DGSVKREDIF YTSKLWCNSH RPELVRPALE RSLKNLQLDY VDLYLIHFPV SVKPGEEVIP KDENGKILFD TVDLCATWEA VEKCKDAGLA KSIGVSNFNR RQLEMILNKP GLKYKPVCNQ VECHPYFNQR KLLDFCKSKD IVLVAYSALG SHREEPWVDP NSPVLLEDPV LCALAKKHKR TPALIALRYQ LQRGVVVLAK SYNEQRIRQN VQVFEFQLTS EEMKAIDGLN RNVRYLTLDI FAGPPNYPFS DEY
Expression System E.coli
Purity > 90% pure
Molecular Weight 38.9 kDa (343aa), confirmed by MALDI-TOF.
Tag/Conjugate His tag
Form & Buffer Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 1mM DTT and 20% glycerol.
Concentration 0.5 mg/ml

Usage & Assay Information

Bioactivity Specific activity: approximately 0.15 - 0.2 units/mg. Enzymatic activity was confirmed by measuring the amount of enzyme catalyzing the oxidation of 1 uM NADPH per minute at 25 DEG C.

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance AKR1C1 is member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the reduction of progesterone to the inactive form 20-alpha-hydroxy-progesterone. Recombinant AKR1C1 protein was expressed in E. coli and purified by using conventional chromatography techniques.

References
Add a Paper

Sorry, but there are no references currently for this product.

Reviews

You need to be logged in to write a review. Please login here

Sorry there are currently no reviews for this product

Images

  • Coomassie Blue stained SDS-PAGE of AKR1C1 protein (His tag) (80R-1267) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: $220.00
Size: 100 ug
OR
Shipping
View Our Distributors