AKR7A2 protein (His tag) (80R-1255)

Purified recombinant Human AKR7A2 protein

Synonyms AKRA2 7 protein, AFB1AR1 protein, aldoketoreductase 7., aflatoxin aldehyde reductase protein, aflatoxin beta1 aldehyde reductase protein, AFAR1 protein, AKR7 protein, member A2 AFB1 AR1 protein, member A2 protein, AKR7A2, aldo keto reductase family 7 protein, AKRA2 7, aldo keto reductase family 7 protein, aldo-keto reductase family 7 protein, AFAR protein, AKRA2-7, Aflatoxin B1 aldehyde reductase member 2 protein, Aiar protein, AFB1-AR1 protein, AKRA2-7 protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE
Assay Information 1. Prepare a 1ml reaction mix in a suitable container: The final concentrations are 0.1M sodium phosphate (pH 7.0), 10mM DL-glyceraldehyde, 0.3mM NADPH. 2. Add 50ul of recombinant AKR7A2 solution with various concentrations (2.5ug, 5ug, 10ug) in 750ul reaction buffer. 3. Mix by inversion and Incubate at 25 DEG C for 2.5 minutes. 4. Add 200ul of 50 mM DL-glyceraldehyde as a substrate and immediately mix by inversion. 5. Record the increase at A340nm for 3 minutes.

Images

Coomassie Blue stained SDS-PAGE of AKR7A2 protein (His tag) (80R-1255)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 1-359 amino acids: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSELEM LSAASRVVSR AAVHCALRSP PPEARALAMS RPPPPRVASV LGTMEMGRRM DAPASAAAVR AFLERGHTEL DTAFMYSDGQ SETILGGLGL GLGGGDCRVK IATKANPWDG KSLKPDSVRS QLETSLKRLQ CPQVDLFYLH APDHGTPVEE TLHACQRLHQ EGKFVELGLS NYASWEVAEI CTLCKSNGWI LPTVYQGMYN ATTRQVETEL FPCLRHFGLR FYAYNPLAGG LLTGKYKYED KDGKQPVGRF FGNSWAETYR NRFWKEHHFE AIALVEKALQ AAYGASAPSV TSAALRWMYH HSQLQGAHGD AVILGMSSLE QLEQNLAATE EGPLEPAVVD AFNQAWHLVA HECPNYFR
Expression System E.coli
Purity > 90% pure
Molecular Weight 44.0 kDa (398aa), confirmed by MALDI-TOF.
Tag/Conjugate His tag
Form & Buffer Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 1mM DTT and 20% glycerol.
Concentration 0.5 mg/ml

Usage & Assay Information

Bioactivity Specific activity: approximately 0.25-0.3 units/mg. Enzymatic activity was confirmed by measuring the amount of enzyme catalyzing the oxidation of 1 uM NADPH per minute at 25 DEG C.

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Aldo-keto reductases, such as AKR7A2, are involved in the detoxification of aldehydes and ketones. This protein can reduce the dialdehyde protein-binding form of aflatoxin B1 (AFB1) to the nonbinding AFB1 dialcohol. It may be involved in protection of liver against the toxic and carcinogenic effects of AFB1, a potent hepatocarcinogen. Also, it has been proposed previously to catalyze the NADPH-dependent reduction of succinic semialdehyde (SSA) to gamma-Hydroxybutyrate in human brain. Recombinant AKR7A2 protein was expressed in E. coli and purified by using conventional chromatography techniques.

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Images

  • Coomassie Blue stained SDS-PAGE of AKR7A2 protein (His tag) (80R-1255) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: $340.00
Size: 100 ug
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