AKR7A3 protein (His tag) (80R-1253)

Purified recombinant Human AKR7A3 protein

Synonyms AFAR2 protein, AKRA3 7, AFB1 AR 2 protein, Aflatoxin B1 aldehyde reductase member 3 protein, Aldo-keto reductase family 7 protein, OTTHUMP00000002623., AFB1 aldehyde reductase 2(AFAR2) protein, Aflatoxin aldehyde reductase protein, AKRA3 7 protein, AKRA3-7 protein, Aflatoxin B1 aldehyde reductase 2 protein, AKRA3-7, member A 3 AFAR 2 protein, AKR7A3, Aldo keto reductase family 7 member A3 protein, Aflatoxin B1 aldehyde reductase member 3 protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE
Assay Information 1. Prepare a 1ml reaction mix into a suitable container: The final concentrations are 0.1M sodium phosphate (pH 7.0), 10mM DL-glyceraldehyde, 0.3mM NADPH. 2. Add 50 ul of recombinant AKR7A3 solution with various concentrations (2.5ug, 5ug, 10ug) in 750 ul reaction buffer. 3. Mix by inversion and Incubate at 25 DEG C for 2.5 minutes. 4. Add 200ul of 50 mM DL-glyceraldehyde as a substrate and immediately mix by inversion. 5. Record the increase at A340nm for 3 minutes.

Images

Coomassie Blue stained SDS-PAGE of AKR7A3 protein (His tag) (80R-1253)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 1-331 amino acids: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSELEM SRQLSRARPA TVLGAMEMGR RMDAPTSAAV TRAFLERGHT EIDTAFVYSE GQSETILGGL GLRLGGSDCR VKIDTKAIPL FGNSLKPDSL RFQLETSLKR LQCPRVDLFY LHMPDHSTPV EETLRACHQL HQEGKFVELG LSNYAAWEVA EICTLCKSNG WILPTVYQGM YNAITRQVET ELFPCLRHFG LRFYAFNPLA GGLLTGKYKY EDKDGKQPVG RFFGNTWAEM YRNRYWKEHH FEGIALVEKA LQAAYGASAP SMTSATLRWM YHHSQLQGAH GDAVILGMSS LEQLEQNLAA AEEGPLEPAV VDAFNQAWHL VAHECPNYFR
Expression System E.coli
Purity > 95% pure
Molecular Weight 41.6 kDa (370aa), confirmed by MALDI-TOF.
Tag/Conjugate His tag
Form & Buffer Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 100mM NaCl and 10% glycerol.
Concentration 0.5 mg/ml

Usage & Assay Information

Bioactivity Specific activity: approximately < 0.1 units/mg. Enzymatic activity was confirmed by measuring the amount of enzyme catalyzing the oxidation of 1 uM NADPH per minute at 25 DEG C.

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Aldo-keto reductase family 7, member A3, also known as AKR7A3, is a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member includes a number of related monomeric NADPH-dependent oxidoreductases, such as aldehyde reductase, aldose reductase, prostaglandin F synthase, xylose reductase, rho crystallin, and many others. AKR7A3 is involved in the detoxification of aldehydes and ketones. The activity of AKR7A3 may detoxify the aflatoxin B1 (AFB1) dialdehyde, which reacts with proteins, and thereby inhibits AFB 1 induced toxicity. Recombinant human AKR7A3, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

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Images

  • Coomassie Blue stained SDS-PAGE of AKR7A3 protein (His tag) (80R-1253) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: $340.00
Size: 50 ug
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