AKT1 antibody (70R-10493)

Affinity purified rabbit polyclonal AKT1 antibody

Synonyms Polyclonal AKT1 antibody, Anti-AKT1 antibody, AKT 1 antibody, AKT 1, AKT-1 antibody, v-akt murine thymoma viral oncogene homolog 1 antibody, AKT-1, AKT1
Cross Reactivity Human, Mouse, Dog, Rat, African Clawed Frog, Chicken, Pig, Bovine
Applications WB
Immunogen AKT1 antibody was raised in rabbit using the N terminal of AKT1 as the immunogen
Assay Information AKT1 Blocking Peptide, catalog no. 33R-8182, is also available for use as a blocking control in assays to test for specificity of this AKT1 antibody

Images

Western blot analysis using AKT1 antibody (70R-10493)

Recommended AKT1 Antibody Titration: 0.2-1 ug/ml

Specifications

Host Rabbit
Form & Buffer Lyophilized powder. Add 50 ul of distilled water. Final antibody concentration is 1 mg/ml in PBS buffer.
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations WB: 0.2-1 ug/ml

Storage & Safety

Storage Store at 4 deg C, following reconstitution, aliquot and store at -20 deg C. Avoid repeated freeze-thaw cycles.

General Information

Biological Significance AKT1 is a general protein kinase capable of phosphorylating several known proteins.The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene.

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Images

  • Western blot analysis using AKT1 antibody (70R-10493) | Recommended AKT1 Antibody Titration: 0.2-1 ug/ml
  • Western blot analysis using AKT1 antibody (70R-10493) | Recommended AKT1 Antibody
  • Western blot analysis using AKT1 antibody (70R-10493) | Lanes:  1. 50 ug xenopus laevis embryos lysate (lysate buffer) 2. 50 ug xenopus laevis embryos lysate (HB buffer) stained with AKT1 antibody at 1:500

Availability: In stock

Price: $345.00
Size: 50 ug
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