AKT2 antibody (10R-3265)

Mouse monoclonal AKT2 antibody

Synonyms Monoclonal AKT2 antibody, Anti-AKT2 antibody, V-akt murine thymoma viral oncogene homolog 2 antibody, Protein kinase Akt-2 antibody, Protein kinase B beta antibody, RAC-PK-beta antibody, PKB beta antibody, PKBB antibody, AKT2, AKT 2, AKT-2, AKT 2 antibody, AKT-2 antibody
Cross Reactivity Human
Applications FC, IF, IHC, WB
Immunogen AKT2 antibody was raised in mouse using a full length recombinant protein of human AKT2 (NP_001617) produced in HEK293T cells, as the immunogen.

Images

Flow Cytometric analysis using AKT2 antibody (10R-3265)

Flow Cytometric analysis of Hela cells using AKT2 antibody (Red), compared to a nonspecific negative control antibody (Blue).

Specifications

Host Mouse
Clone 4H7
Isotype IgG1
Method of Purification AKT2 antibody was purified by affinity chromatography.
Form & Buffer Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Concentration 500 ug-1 mg/ml

Usage & Assay Information

Usage Recommendations FC: 1:100, IF: 1:100, IHC: 1:50, WB: 1:1000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance AKT2 is a member of a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. The gene was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. The encoded protein is a general protein kinase capable of phophorylating several known proteins.

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Images

  • Flow Cytometric analysis using AKT2 antibody (10R-3265) | Flow Cytometric analysis of Hela cells using AKT2 antibody (Red), compared to a nonspecific negative control antibody (Blue).
  • Western Blot analysis using AKT2 antibody (10R-3265) | Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant AKT2 protein (Right) or empty vector (Left) detected with AKT2 antibody
  • Immunohistochemical staining using AKT2 antibody (10R-3265) | Immunohistochemical analysis of AKT2 protein in paraffin embedded Human liver tissue using AKT2 antibody
  • Immunofluorescent staining using AKT2 antibody (10R-3265) | Immunofluorescent staining of COS7 cells transiently transfected with recombinant AKT2 protein using AKT2 antibody

Availability: In stock

Price: $455.00
Size: 100 ul
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