AKT3 antibody (10R-3271)

Mouse monoclonal AKT3 antibody

Synonyms Monoclonal AKT3 antibody, Anti-AKT3 antibody, V-akt murine thymoma viral oncogene homolog 3 antibody, PKBG antibody, PRKBG antibody, Protein kinase Akt 3 antibody, Protein kinase B gamma antibody, RAC PK gamma antibody, STK2 antibody, Serine threonine protein kinase Akt 3 antibody, AKT3, AKT 3, AKT-3, AKT 3 antibody, AKT-3 antibody
Cross Reactivity Human, Mouse
Applications IF, IHC, WB
Immunogen AKT3 antibody was raised in mouse using a recombinant fragment expressed in E. coli corresponding to amino acids 2-479 of human AKT3 as the immunogen.

Images

Immunofluorescent staining using AKT3 antibody (10R-3271)

Immunofluorescent staining of Hela cells transiently transfected with recombinant AKT3 protein using AKT3 antibody

Specifications

Host Mouse
Clone 9H8
Isotype IgG3
Method of Purification AKT3 antibody was purified by affinity chromatography.
Form & Buffer Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Concentration 500 ug-1 mg/ml

Usage & Assay Information

Usage Recommendations IF: 1:100, IHC: 1:50, WB: 1:500

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance AKT3 is a member of the AKT, also called PKB, serine/threonine protein kinase family. AKTkinases are known to be regulators of cell signaling in response to insulin and growth factors. They are involved in awide variety of biological processes including cell proliferation, differentiation, apoptosis, tumorigenesis, as wellas glycogen synthesis and glucose uptake. This kinase has been shown to be stimulated by platelet-derived growthfactor (PDGF), insulin, and insulin-like growth factor 1 (IGF1).

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Images

  • Immunofluorescent staining using AKT3 antibody (10R-3271) | Immunofluorescent staining of Hela cells transiently transfected with recombinant AKT3 protein using AKT3 antibody
  • Western Blot analysis using AKT3 antibody (10R-3271) | Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant AKT3 protein (Right) or empty vector (Left) detected with AKT3 antibody
  • Immunohistochemical staining using AKT3 antibody (10R-3271) | Immunohistochemical analysis of AKT3 protein in paraffin embedded Carcinoma of Human lung tissue using AKT3 antibody

Availability: In stock

Price: $395.00
Size: 100 ul
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