ALDOB antibody (10R-3297)

Mouse monoclonal ALDOB antibody

Synonyms Monoclonal ALDOB antibody, Anti-ALDOB antibody, Aldolase B Fructose-Bisphosphate antibody, ALDB antibody, Fructose-bisphosphate aldolase B antibody
Cross Reactivity Human
Applications FC, IHC, WB
Immunogen ALDOB antibody was raised in mouse using a full length recombinant protein of human ALDOB (NP_000026) produced in HEK293 cell as the immunogen.

Images

Flow Cytometric analysis using ALDOB antibody (10R-3297)

Flow Cytometric analysis of HEK293T cells transfected with either recombinant ALDOB protein (red) or empty vector (blue) stained using ALDOB antibody

Specifications

Host Mouse
Clone 3C6
Isotype IgG2b
Method of Purification ALDOB antibody was purified by affinity chromatography.
Form & Buffer Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Concentration 500 ug-1 mg/ml

Usage & Assay Information

Usage Recommendations FC: 1:100, IHC: 1:150, WB: 1:2000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance ALDOB is a tetrameric glycolytic enzyme that catalyzes the reversible conversion of fructose-1, 6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Vertebrates have 3 aldolase isozymes which are distinguished by their electrophoretic and catalytic properties. Differences indicate that aldolases A, B, and C are distinct proteins, the products of a family of related 'housekeeping' genes exhibiting developmentally regulated expression of the different isozymes. The developing embryo produces aldolase A, which is produced in even greater amounts in adult muscle where it can be as much as 5% of total cellular protein. In adult liver, kidney and intestine, aldolase A expression is repressed and aldolase B is produced. In brain and other nervous tissue, aldolase A and C are expressed about equally.

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Images

  • Flow Cytometric analysis using ALDOB antibody (10R-3297) | Flow Cytometric analysis of HEK293T cells transfected with either recombinant ALDOB protein (red) or empty vector (blue) stained using ALDOB antibody
  • Western Blot analysis using ALDOB antibody (10R-3297) | Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant ALDOB protein (Right) or empty vector (Left) detected with ALDOB antibody
  • Immunohistochemical staining using ALDOB antibody (10R-3297) | Immunohistochemical analysis of ALDOB protein in paraffin embedded Human liver tissue using ALDOB antibody

Availability: In stock

Price: $455.00
Size: 100 ul
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